Prostaglandin dehydrogenase 1 (HPGD) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1C10]

CAT#: TA502450BM

HPGD (Prostaglandin dehydrogenase 1) mouse monoclonal antibody, clone OTI1C10 (formerly 1C10), HRP conjugated

Product Images

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human HPGD plasmid (RC204160, hHPGD), mouse HPGD plasmid (MR203530, mHPGD) using anti-HPGD antibody TA502450(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HPGD mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  HEK293T cells transfected with either RC204160 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HPGD antibody (TA502450), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1C10
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HPGD (NP_000851) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.8 kDa
• Gene Name: 15-hydroxyprostaglandin dehydrogenase
• Database Link:
  NP_000851
  Entrez Gene 3248 Human
  UniProt ID P15428
• Background: This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
• Synonyms: 15-PGDH; PGDH; PGDH1; PHOAR1; SDR36C1

Reference Data

• Protein Families: Druggable Genome