RTN4IP1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1B2]

CAT#: TA502543BM

RTN4IP1 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTN4IP1 (RC202957, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1. Positive lysates LY409966 (100ug) and LC409966 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RTN4IP1 monoclonal antibody.

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  HEK293T cells transfected with either RC202957 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RTN4IP1 antibody (TA502543), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-RTN4IP1 antibody (TA502543), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-RTN4IP1 antibody (TA502543), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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Specifications

Product Data

• Clone Name: OTI1B2
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RTN4IP1 (NP_116119) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.9 kDa
• Gene Name: reticulon 4 interacting protein 1
• Database Link:
  NP_116119
  Entrez Gene 170728 MouseEntrez Gene 309912 RatEntrez Gene 84816 Human
  UniProt ID Q8WWV3
• Background: This gene encodes a novel mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. The interaction of reticulon 4 with mitochondrial proteins may provide insight into the mechanisms for reticulon-induced inhibition of neurite growth. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: NIMP; OPA10

Reference Data

• Protein Families: Druggable Genome