Prostaglandin dehydrogenase 1 (HPGD) Mouse Monoclonal Antibody [Clone ID: OTI2C10]

CAT#: TA502561

HPGD (Prostaglandin dehydrogenase 1) mouse monoclonal antibody, clone OTI2C10 (formerly 2C10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPGD (RC204160, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPGD. Positive lysates LY400305 (100ug) and LC400305 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502561)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502561)

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  HEK293T cells transfected with either RC204160 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HPGD antibody (TA502561), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-HPGD antibody (TA502561), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-HPGD antibody (TA502561), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI2C10
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HPGD (NP_000851) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.8 kDa
• Gene Name: 15-hydroxyprostaglandin dehydrogenase
• Database Link:
  NP_000851
  Entrez Gene 3248 Human
  UniProt ID P15428
• Background: This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
• Synonyms: 15-PGDH; PGDH; PGDH1; PHOAR1; SDR36C1

Reference Data

• Protein Families: Druggable Genome