PNPO Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2F8]

CAT#: TA503178BM

PNPO mouse monoclonal antibody, clone OTI2F8 (formerly 2F8), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (RC200133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO. Positive lysates LY413282 (100ug) and LC413282 (20ug) can be purchased separately from OriGene.

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  Anti-PNPO mouse monoclonal antibody (TA503178) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNPO (RC200133).

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  HEK293T cells transfected with either RC200133 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PNPO antibody (TA503178), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2F8
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PNPO(NP_060599) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.8 kDa
• Gene Name: pyridoxamine 5'-phosphate oxidase
• Database Link:
  NP_060599
  Entrez Gene 64533 RatEntrez Gene 103711 MouseEntrez Gene 55163 Human
  UniProt ID Q9NVS9
• Background: The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: HEL-S-302; PDXPO

Reference Data

• Protein Pathways: Metabolic pathways, Vitamin B6 metabolism