RGS16 Mouse Monoclonal Antibody [Clone ID: OTI4E5]

CAT#: TA504031

RGS16 mouse monoclonal antibody, clone OTI4E5 (formerly 4E5)

Product Images

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human RGS16 plasmid (RC202430, lane 2), mouse RGS16 plasmid (MR202050, lane 3) using anti-RGS16 antibody TA504031 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Western blot analysis of extracts (10ug) from a mouse tissue by using anti-RGS16 monoclonal antibody (1:200).

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  Western blot analysis of extracts (50ug) from Jurkat lysate by using anti-RGS16 monoclonal antibody(TA504031, 1:500)

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RGS16 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RGS16 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RGS16 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RGS16 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-RGS16 mouse monoclonal antibody (TA504031) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RGS16 (RC202430).

• Flow cytometric Analysis of Jurkat cells, using anti-RGS16 antibody (TA504031), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI4E5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RGS16(NP_002919) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.6 kDa
• Gene Name: regulator of G protein signaling 16
• Database Link:
  NP_002919
  Entrez Gene 6004 Human
  UniProt ID O15492
• Background: The protein encoded by this gene belongs to the 'regulator of G protein signaling' family. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. [provided by RefSeq]
• Synonyms: A28-RGS14; A28-RGS14P; RGS-R

Reference Data

• Protein Families: Druggable Genome