DGKA Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI6A11]

CAT#: TA504050AM

DGKA mouse monoclonal antibody, clone OTI6A11 (formerly 6A11), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKA (RC222395, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKA. Positive lysates LY400535 (100ug) and LC400535 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-DGKA monoclonal antibody (1:200).

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  Flow cytometric Analysis of Jurkat cells, using anti-DGKA antibody (TA504050), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI6A11
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DGKA(NP_001336) produced in HEK293 cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.5 kDa
• Gene Name: diacylglycerol kinase alpha
• Database Link:
  NP_001336
  Entrez Gene 1606 Human
  UniProt ID P23743
• Background: The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq]
• Synonyms: DAGK; DAGK1; DGK-alpha

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system