NQO2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3G4]

CAT#: TA504781AM

NQO2 mouse monoclonal antibody,clone 3G4, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NQO2 (Cat# RC202889, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NQO2(Cat# TA504781). Positive lysates LY424463 (100ug) and LC424463 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-NQO2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NQO2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NQO2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NQO2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NQO2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NQO2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NQO2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NQO2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NQO2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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Specifications

Product Data

• Clone Name: OTI3G4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Dog, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NQO2(NP_000895) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.7 kDa
• Gene Name: N-ribosyldihydronicotinamide:quinone reductase 2
• Database Link:
  NP_000895
  Entrez Gene 18105 MouseEntrez Gene 606932 DogEntrez Gene 707675 MonkeyEntrez Gene 4835 Human
  UniProt ID P16083
• Background: NQO2 (EC 1.10.99.2) is a flavoprotein that catalyzes the 2-electron reduction of various quinones, redox dyes, and the vitamin K menadione. NQO2 predominantly uses dihydronicotinamide riboside (NRH) as the electron donor (summary by Wu et al., 1997 [PubMed 9367528]). [supplied by OMIM]
• Synonyms: DHQV; DIA6; NMOR2; QR2