NONO Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3G4]

CAT#: TA504847BM

NONO mouse monoclonal antibody,clone 3G4, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (RC206688, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO. Positive lysates LY402135 (100ug) and LC402135 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT, Cat# LC810293T) and NONO-Knockout HEK293T cells (KO, Cat# LC841199) were separated by SDS-PAGE and immunoblotted with anti-NONO monoclonal antibody TA504847 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI3G4
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 184-385 of human NONO(NP_031389) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.1 kDa
• Gene Name: non-POU domain containing octamer binding
• Database Link:
  NP_031389
  Entrez Gene 53610 MouseEntrez Gene 317259 RatEntrez Gene 612773 DogEntrez Gene 697263 MonkeyEntrez Gene 4841 Human
  UniProt ID Q15233
• Background: This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
• Synonyms: MRXS34; NMT55; NRB54; P54; P54NRB; PPP1R114

Reference Data

• Protein Families: Druggable Genome, Transcription Factors