MAGEA4 Mouse Monoclonal Antibody [Clone ID: OTI2C1]

CAT#: TA505361

MAGEA4 mouse monoclonal antibody, clone OTI2C1 (formerly 2C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA4 (RC223991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA4. Positive lysates LY423276 (100ug) and LC423276 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505361)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505361)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505361)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505361)

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  Anti-MAGEA4 mouse monoclonal antibody (TA505361) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAGEA4 (RC223991).

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Specifications

Product Data

• Clone Name: OTI2C1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MAGEA4(NP_001011550) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.7 kDa
• Gene Name: MAGE family member A4
• Database Link:
  NP_001011550
  Entrez Gene 4103 Human
  UniProt ID P43358
• Background: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq]
• Synonyms: CT1.4; MAGE-41; MAGE-X2; MAGE4; MAGE4A; MAGE4B