PCDH7 Mouse Monoclonal Antibody [Clone ID: OTI2G6]

CAT#: TA505452S

PCDH7 mouse monoclonal antibody, clone OTI2G6 (formerly 2G6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCDH7 (RC223033, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCDH7. Positive lysates LY410093 (100ug) and LC410093 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PCDH7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PCDH7 monoclonal antibody (1:200).

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  Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-PCDH7 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PCDH7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505452)

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  Anti-PCDH7 mouse monoclonal antibody (TA505452) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PCDH7 (RC223033).

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Specifications

Product Data

• Clone Name: OTI2G6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PCDH7(NP_115833) produced in HKE293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 132.4 kDa
• Gene Name: protocadherin 7
• Database Link:
  NP_115833
  Entrez Gene 54216 MouseEntrez Gene 488843 DogEntrez Gene 696011 MonkeyEntrez Gene 5099 Human
  UniProt ID O60245
• Background: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a protein with an extracellular domain containing 7 cadherin repeats. The gene product is an integral membrane protein that is thought to function in cell-cell recognition and adhesion. Alternative splicing yields isoforms with unique cytoplasmic tails. [provided by RefSeq, Jul 2008]
• Synonyms: BH-Pcdh; BHPCDH; PPP1R120

Reference Data

• Protein Families: Druggable Genome, Transmembrane