CK1 epsilon (CSNK1E) Mouse Monoclonal Antibody [Clone ID: OTI5D4]

CAT#: TA803799S

CSNK1E mouse monoclonal antibody, clone OTI5D4 (formerly 5D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSNK1E (RC202436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSNK1E. Positive lysates LY403455 (100ug) and LC403455 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and CSNK1E-Knockout Hela cells (KO, Cat# LC810054) were separated by SDS-PAGE and immunoblotted with anti-CSNK1E monoclonal antibody TA803799. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-CSNK1E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803799)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CSNK1E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803799)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CSNK1E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803799)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CSNK1E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803799)

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Specifications

Product Data

• Clone Name: OTI5D4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 153-416 of human CSNK1E (NP_689407) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.1 kDa
• Gene Name: casein kinase 1 epsilon
• Database Link:
  NP_689407
  Entrez Gene 27373 MouseEntrez Gene 58822 RatEntrez Gene 1454 Human
• Synonyms: CKIepsilon; HCKIE

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Circadian rhythm - mammal, Hedgehog signaling pathway, Wnt signaling pathway