Neurofilament (NEFM) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5H4]

CAT#: TA805947BM

NEFM mouse monoclonal antibody, clone OTI5H4 (formerly 5H4), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFM (RC224475, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFM. Positive lysates LY417338 (100ug) and LC417338 (20ug) can be purchased separately from OriGene.

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and NEFM-Knockout 293T cells (KO, Cat# LC810526) were separated by SDS-PAGE and immunoblotted with anti-NEFM monoclonal antibody TA805947, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

Specifications

Product Data

• Clone Name: OTI5H4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 413-773 of human NEFM(NP_005373) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: neurofilament, medium polypeptide
• Database Link:
  NP_005373
  Entrez Gene 4741 Human
  UniProt ID P07197
• Synonyms: NEF3; NF-M; NFM

Reference Data

• Protein Pathways: Amyotrophic lateral sclerosis (ALS)