INDOL1 (IDO2) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4B3]

CAT#: TA806162BM

IDO2 mouse monoclonal antibody, clone OTI4B3 (formerly 4B3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (RC223337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2. Positive lysates LY403659 (100ug) and LC403659 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines and 4 human tissue by using anti-IDO2 monoclonal antibody (1: Hela; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus) at 1:200 dilution.

Specifications

Product Data

• Clone Name: OTI4B3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IDO2 (NP_919270) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.9 kDa
• Gene Name: indoleamine 2,3-dioxygenase 2
• Database Link:
  NP_919270
  Entrez Gene 169355 Human
  UniProt ID Q6ZQW0
• Background: Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]). [supplied by OMIM, Feb 2011]
• Synonyms: INDOL1

Reference Data

• Protein Pathways: Metabolic pathways, Tryptophan metabolism