Clusterin (CLU) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5D3]

CAT#: TA808665BM

CLU mouse monoclonal antibody, clone OTI5D3 (formerly 5D3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLU (RC203941, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLU (1:2000). Positive lysates LY404400 (100ug) and LC404400 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CLU monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808665) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808665) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808665) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808665) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808665) (1:150)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808665) (1:150)

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Specifications

Product Data

• Clone Name: OTI5D3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 23-227 of human CLU(NP_976084) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: clusterin
• Database Link:
  NP_976084
  Entrez Gene 1191 Human
  UniProt ID P10909
• Synonyms: AAG4; APOJ; CLI; KUB1; MGC24903; SGP-2; SGP2; SP-40; TRPM-2; TRPM2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein