Vitamin D Binding protein (GC) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2A1]

CAT#: TA809264AM

GC mouse monoclonal antibody,clone OTI2A1, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GC (RC208864, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GC (1:2000). Positive lysates LY400197 (100ug) and LC400197 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-GC monoclonal antibody (HeLa: human; 293T: human; T47D: human) (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809264) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809264) (1:150)

Specifications

Product Data

• Clone Name: OTI2A1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GC (NP_000574) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.1 kDa
• Gene Name: GC, vitamin D binding protein
• Database Link:
  NP_000574
  Entrez Gene 2638 Human
  UniProt ID P02774
• Background: The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
• Synonyms: DBP; GC; Gc-MAF; GcMAF; GRD3; HEL-S-51; VDBG; VDBP

Reference Data

• Protein Families: Secreted Protein