PITPNB Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5D11]

CAT#: TA809354BM

PITPNB mouse monoclonal antibody,clone OTI5D11, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PITPNB (RC204262, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PITPNB (1:2000). Positive lysates LY415778 (100ug) and LC415778 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-PITPNB monoclonal antibody (A431: human; 293T: human; Jurkat: human) (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and PITPNB-Knockout Hela cells (KO, Cat# LC810341) were separated by SDS-PAGE and immunoblotted with anti-PITPNB monoclonal antibody TA809354, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PITPNB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809354) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PITPNB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809354) (1:2000)

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Specifications

Product Data

• Clone Name: OTI5D11
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PITPNB(NP_036531) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.4 kDa
• Gene Name: phosphatidylinositol transfer protein beta
• Database Link:
  NP_036531
  Entrez Gene 56305 MouseEntrez Gene 114561 RatEntrez Gene 23760 Human
  UniProt ID P48739
• Background: This gene encodes a cytoplasmic protein that catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes. This transfer activity is required for COPI complex-mediated retrograde transport from the Golgi apparatus to the endoplasmic reticulum. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]
• Synonyms: PI-TP-beta; PtdInsTP; VIB1B