ABCF2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI8F4]

CAT#: TA811105AM

ABCF2 mouse monoclonal antibody,clone OTI8F4, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCF2 (RC200323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCF2 (1:2000). Positive lysates LY402100 (100ug) and LC402100 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-ABCF2 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and ABCF2-Knockout 293T cells (KO, Cat# LC811907) were separated by SDS-PAGE and immunoblotted with anti-ABCF2 monoclonal antibody TA811105, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811105) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811105) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811105) (1:500)

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Specifications

Product Data

• Clone Name: OTI8F4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-235 of human ABCF2 (NP_009120) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71.1 kDa
• Gene Name: ATP binding cassette subfamily F member 2
• Database Link:
  NP_009120
  Entrez Gene 27407 MouseEntrez Gene 10061 Human
  UniProt ID Q9UG63
• Synonyms: ABC28; EST133090; HUSSY-18; HUSSY18

Reference Data

• Protein Families: Druggable Genome