EEA1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2G7]

CAT#: TA813373AM

EEA1 mouse monoclonal antibody, clone OTI2G7, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EEA1 (RC215396, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EEA1.(1:1000)

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-EEA1 monoclonal antibody. (1:500)

Specifications

Product Data

• Clone Name: OTI2G7
• Applications: WB
• Recommended Dilution: WB 1:500-1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-400 of human EEA1 (NP_003557) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 162.3 kDa
• Gene Name: early endosome antigen 1
• Database Link:
  NP_003557
  Entrez Gene 216238 MouseEntrez Gene 314764 RatEntrez Gene 8411 Human
  UniProt ID Q15075
• Background: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. [UniProtKB/Swiss-Prot Function]
• Synonyms: MST105; MSTP105; ZFYVE2

Reference Data

• Protein Pathways: Endocytosis