L1CAM Mouse Monoclonal Antibody [Clone ID: UMAB48]

CAT#: UM570044

L1CAM mouse monoclonal antibody, clone UMAB48

Product Images

Western blot analysis of extracts (35ug) from 3 cell line lysates by using anti-L1CAM monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and L1CAM-Knockout HeLa cells (KO, Cat# LC832196 ) were separated by SDS-PAGE and immunoblotted with anti-L1CAM monoclonal antibody UM500044 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-L1CAM mouse monoclonal antibody. (UM500044; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-L1CAM mouse monoclonal antibody. (UM500044; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded human pituitary using anti-L1CAM clone UMAB48 mouse monoclonal antibody (UM500044) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows only a subset of cells with positive cytoplasmic and membraneous stain.

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  Immunohistochemical staining of paraffin-embedded human colon cancer using anti-L1CAM clone UMAB48 mouse monoclonal antibody (UM500044) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows tumor cells with positive cytoplasmic and membraneous stain.

• Immunohistochemical staining of paraffin-embedded human melanoma using anti-L1CAM clone UMAB48 mouse monoclonal antibody (UM500044) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows strong positive membraneous and cytoplasmic staining in the tumor cells.

• Immunohistochemical staining of paraffin-embedded human brain using anti-L1CAM clone UMAB48 mouse monoclonal antibody (UM500044) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows positive cytoplasmic and membraneous stain granular layer and molecular layer.

• Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-L1CAM clone UMAB48 mouse monoclonal antibody (UM500044) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows positive cytoplasmic and membraneous stain in a subset of the tumor cells.

• Confocal immunofluoresce image of primary rat neurons labeled with anti-L1CAM mouse monoclonal antibody (UM500044, green, 1:50). Actin filaments were labeled with TRICT-Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

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Specifications

Product Data

• Clone Name: UMAB48
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human L1CAM (NP_000416) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 137.8 kDa
• Gene Name: Homo sapiens L1 cell adhesion molecule (L1CAM), transcript variant 1, mRNA.
• Database Link:
  NP_000416
  Entrez Gene 16728 MouseEntrez Gene 50687 RatEntrez Gene 3897 Human
  UniProt ID P32004
• Background: The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq, Jul 2008]
• Synonyms: CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Axon guidance, Cell adhesion molecules (CAMs)