PD1 (PDCD1) Mouse Monoclonal Antibody [Clone ID: UMAB222]

CAT#: UM800114CF

Carrier-free (BSA/glycerol-free) PD-1 (PDCD1) mouse monoclonal antibody, clone UMAB222

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1 antibody (1:2000).

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  Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody (UM800114) (Red) compared to a nonspecific negative control antibody (Blue) (1:50).

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  HEK293T cells transfected with either mouse PD1 (MR227347) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDCD1 antibody (UM800114), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry (1:50).

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PDCD1 mouse monoclonal antibody (UM800114). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

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  Flow cytometric Analysis of stable expression PDL1 (RC213071) cells using anti-PDCD1 antibody (UM800114) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc (human) IgG-FITC (1:50).

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  Flow cytometric Analysis of PDL2 (RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody (UM800114) (blue) or 1ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc (human) IgG-FITC (1:50).

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Specifications

Product Data

• Clone Name: UMAB222
• Applications: 10k-ChIP, FC, NEUT, WB
• Recommended Dilution: IHC 1:100, FLOW 1:50
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDCD1 (NP_005009) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.2 kDa
• Gene Name: Homo sapiens programmed cell death 1 (PDCD1), mRNA.
• Database Link:
  NP_005009
  Entrez Gene 18566 MouseEntrez Gene 5133 Human
  UniProt ID Q15116
• Background: This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
• Synonyms: CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), T cell receptor signaling pathway