Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose Beads

CAT#: TA150037

Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose Beads

Product Images

Immunoprecipitation was performed with HEK293T cell lysates of expressing Entry vector (control, Cat# PS100001) or expressing DDK-tagged PON3 (Cat# LY400339). Anti-DDK 4C5-agarose beads were incubated with control lysate or LY400339 overnight at 4C respectively. After washing three times with lysate buffer, 20uL 4x SDS sample buffer was applied. The samples were boiled at 95°C for 10min. The supernatant was loaded to the SDS-PAGE gel after discarded the beads by centrifuging. After transferring to the membrane, the Western blot assay was applied with HRP-conjugated anti-DDK antibody (Cat# TA150030). Lane 1: control lysate; Lane 2: DDK-tagged PON3 (LY400339); Lane 3: anti-DDK 4C5 agarose beads (TA150037) 50uL with control lysate; Lane 4: anti-DDK 4C5 agarose beads (TA150037) 50uL with LY400339.

Specifications

Product Data

• Clone Name: OTI4C5 (formerly 4C5)
• Applications: IP, Protein Purification
• Recommended Dilution: IP, Protein Purification
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH
• Isotype: IgG2a
• Formulation: PBS (pH7.4) containing 0.1% BSA, 0.02% NaN3 and 50% glycerol
• Conjugation: Agarose
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Synonyms: ANTI DDK, ANTI-DDK, ANTIDDK, FLAG TAG, ANTI FLAG, ANTI-FLAG, ANTI FLAG ANTIBODY, FLAG ANTIBODY, M2 ANTIBODY, FLAG M2, ANTIBODY FOR FLAG, 4C5, ANTI DDK 4C5, DDK 4C5, ANTI-DDK 4C5, DYKDDDDK TAG, D-TAG ANTIBODY, ANTI D-TAG, ANTI D TAG, ANTI-D TAG, ANTI-MDDK, ANTI-MYC DDK, ANTI-MYC/DDK, MYC/DDK ANTIBODY, MYC DDK ANTIBODY, ANTI MYC DDK, ANTI DDK MYC, ANTI-DDK/MYC, ANTI-DDK MYC, FLAG AB, FLAG MAB, DDK ANTIBODY, FLAG AGAROSE, ANTI FLAG AGAROSE, ANTI FLAG BEAD, ANTI FLAG RESIN, ANTI-FLAG BEAD, ANTI-FLAG RESIN, ANTI-DDK RESIN, ANTI-DDK BEAD, ANTI-DDK COLUMN, ANTI-FLAG COLUMN
• Note: This antibody doesn't recognize N- terminus DDK tag. Please use TA180144 for the detection of DDK tag at any location in Western blotting.