Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CAT#: TA50011-5

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

Product Images

Immunoblot analysis of randomly picked 7 different Myc/DDK tagged overexpression lysates and Myc/DDK tagged Western molecular weight marker (MWM1001) with TA50011 at 1:2,000.

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  Hela cells transiently transfected with Myc/DDK tagged GFAP ORF cDNA clone were immunostained with 4C5 anti-DDK antibody (TA50011, 1:1000), and then colored in green with Alexa 488 conjugated secondary antibody (1:2000). The nuclei (blue) were counterstained with DAPI.

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  Protein purification of a human protein from over-expression lysate with anti-DDK antibody affinity column (TA50011, clone 4C5)

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  HEK293T cells transfected with either Myc/DDK tagged PROM2 ORF overexpression plasmid (red) or empty vector control plasmid (blue) were immunostained with OriGene anti-DDK antibody (TA50011, 1:1000) and analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4C5 (formerly 4C5)
• Applications: FC, IF, IP, WB
• Recommended Dilution: WB: 1:2000, IP: 1:200, IF 1:1000, FC: 1:1000
• Target: DDK (FLAG tag): DYKDDDDK
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH
• Isotype: IgG2a
• Formulation: PBS (pH7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Storage: Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.
• Synonyms: ANTI DDK, ANTI-DDK, ANTIDDK, FLAG TAG, ANTI FLAG, ANTI-FLAG, ANTI FLAG ANTIBODY, FLAG ANTIBODY, M2 ANTIBODY, FLAG M2, ANTIBODY FOR FLAG, 4C5, ANTI DDK 4C5, DDK 4C5, ANTI-DDK 4C5, DYKDDDDK TAG, D-TAG ANTIBODY, ANTI D-TAG, ANTI D TAG, ANTI-D TAG, ANTI-MDDK, ANTI-MYC DDK, ANTI-MYC/DDK, MYC/DDK ANTIBODY, MYC DDK ANTIBODY, ANTI MYC DDK, ANTI DDK MYC, ANTI-DDK/MYC, ANTI-DDK MYC, FLAG AB, FLAG MAB, DDK ANTIBODY
• Note: This antibody doesn't recognize N- terminus DDK tag. Please use TA180144 for the detection of DDK tag at any location in Western blotting.