MBD4 (NM_003925) Human 3' UTR Clone

CAT#: SC207386

3`UTR clone of methyl-CpG binding domain protein 4 (MBD4) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MBD4
• Synonyms: MED1
• ACCN: NM_003925
• Insert Size: 594
• Sequence Data:
  Insert Sequence

  >SC207386 3'UTR clone of NM_003925
  The sequence shown below is from the reference sequence of NM_003925. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGACTGGCTTTGGGAAAATCATGAAAAATTAAGTCTATCTTAAACTCTGCAGCTTTCAAGCTCATCTGTT
  ATGCATAGCTTTGCACTTCAAAAAAGCTTAATTAAGTACAACCAACCACCTTTCCAGCCATAGAGATTTT
  AATTAGCCCAACTAGAAGCCTAGTGTGTGTGCTTTCTTAATGTGTGTGCCAATGGTGGATCTTTGCTACT
  GAATGTGTTTGAACATGTTTTGAGATTTTTTTAAAATAAATTATTATTTGACAACAATCCAAAAAAAATA
  CGGCTTTTCCAATGATGAAATATAATCAGAAGATGAAAAATAGTTTTAAACTATCAATAATACAAAGCAA
  ATTTCTATCAGCCTTGCTAAAGCTAGGGGCCCACTAAATATTTTTATCGGCTAGGCGTGGTGGTGCATGC
  CTGTAATCTCGGAAGGCTGAGGCAGGAGGATCATTTGAGCTCATGAGGGCCCAGGAGGTCAAGGCTTCAG
  TGAGCCATGATCATGCCACTGCACTCCAGTCTGGATGACAGAGAGAGACCCTGTCTCAAAAAATATATAT
  TTAAAAAATAAAAATAAAAGCTGACCCCAAAGAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_003925.1
• Summary: The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
• Locus ID: 8930