PPAR delta (PPARD) (NM_177435) Human 3' UTR Clone

CAT#: SC208302

3`UTR clone of peroxisome proliferator-activated receptor delta (PPARD) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PPARD
• Synonyms: FAAR; NR1C2; NUC1; NUCI; NUCII; PPARB
• ACCN: NM_177435
• Insert Size: 621 bp
• Sequence Data:
  Insert Sequence

  >SC208302 3'UTR clone of NM_177435
  The sequence shown below is from the reference sequence of NM_177435. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCATCATTCTGTGTGGAGGTGAGTGAGAGTGGGGCAGGTGGGCTGGCCTGGCACACCCAGTCGTCCTGG
  GGGTTGGCCCTCACTGCAGGGCACTGTGCCTGAGCTCTGACAGTGTGGGGAAGTGTCCCTGTGATCTTGG
  CAGTGGAACATGCAAGGCACTGACTGAGCATGCAGGATCAGCTCCATCTCATTATGTACGTAGATAGAGG
  TGGAGACAGGAAAAAGACTAAGCCAGACGTGGTGGCTCACACCTGTAATCCCAGCACTTTGGCAGGCCGA
  GGCGGGTGGATCACTTGAGGTCAGGAGTTCGAAACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACT
  AAAAATACAAAAAATTAGCCAGATGTGGTGGCACGCGCCTGTAATCCCAGCTACTTGGGAGGCTGAGCCA
  GGAGAATCGCTTGAACCCGAGAGGTGGAGGTTGCAGTGAGCCAAAATCCCACCACTGCACTCCAGCCTGG
  GTGACAGAGTGAGACCCTGTCTCAAAAAAAAGGAAAAGGACTAACAGGCAGTATGCTGTCATGTTAATGT
  GGGGTGGAAAAATTGTCTGCATTTTTTCTGCATTTTTAAAATTCCAACACAATAAATACAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_177435.2
• Summary: 'This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. The encoded protein is thought to function as an integrator of transcriptional repression and nuclear receptor signaling. It may inhibit the ligand-induced transcriptional activity of peroxisome proliferator activated receptors alpha and gamma, though evidence for this effect is inconsistent. Expression of this gene in colorectal cancer cells may be variable but is typically relatively low. Knockout studies in mice suggested a role for this protein in myelination of the corpus callosum, lipid metabolism, differentiation, and epidermal cell proliferation. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Aug 2017]'
• Locus ID: 5467