U2AF65 (U2AF2) (NM_007279) Human 3' UTR Clone

CAT#: SC208640

3`UTR clone of U2 small nuclear RNA auxiliary factor 2 (U2AF2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: U2AF2
• Synonyms: U2AF65
• ACCN: NM_007279
• Insert Size: 652
• Sequence Data:
  Insert Sequence

  >SC208640 3'UTR clone of NM_007279
  The sequence shown below is from the reference sequence of NM_007279. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCGACTCTTATCACCGCCGGGACTTCTGGTAGAGGCGGCTGGGGGAGGGTGGGGGCAGGGCTGGCTGGGG
  GCTTCTCCCCACTCCCGCCCCCCCCTTATCCCCCTCTGAAGACGATGGGCAGAGGAGTGACAGCCGCAGA
  CACACGACAGCCGGCAGCAACTGGAATGGCAGCAATTAAGGGTGGGGGGCGGGGGTTGGGGGGTTGGGGG
  GTTAGGGCAGGGAGGGGACTGGGGAAGTGCGCACACAGCCCACACAGACAACACGCACCCACACAGACAC
  AGAGGGAAGGGGTTGGGATGGGGACAGGGTGCACAGCAGGGCGGGGTAGGACCCCAGCCCCTCCCAAAAC
  AGCCTCTCCTTCTCCCATAGACCCCTTTCTTCTCCCCTTCCCCACGGTAGGAACATAGCGTGTTTATATT
  TTATGGCCAAACTATTTTGAATTTTGTTGTCCGGCCCTCAGTGCCCTGCCCTCTCCCTTACCAGGACCAC
  AGCTCTGTTCCTTCGGCCTCTGGTCCTCTCTGGTCCCCTCCTGGGTTTCTTACGTAGTTGATTTTTCCTC
  TTTAGTCTCCCCCGACCTGCGCCCAGCCCCGTGGCCCCTGCCCCTCTCCTACTCTCTGTGGCAGTTTCAT
  ATTTGCTAAGACGAATTTGCTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_007279.2
• Summary: U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008]
• Locus ID: 11338