PRAS40 (AKT1S1) (NM_032375) Human 3' UTR Clone

CAT#: SC210859

3`UTR clone of AKT1 substrate 1 (proline-rich) (AKT1S1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AKT1S1
• Synonyms: Lobe; PRAS40
• ACCN: NM_032375
• Insert Size: 898
• Sequence Data:
  Insert Sequence

  >SC210859 3'UTR clone of NM_032375
  The sequence shown below is from the reference sequence of NM_032375. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TAACACCAGCGACTTCCAGAAGCTGAAGCGGAAATATTGAAGTCCAGGGAGGGAGCGCCCCGGGCCGCGT
  CCGCCCCGTCCCACACTACGCCCCCGCCCCACTCCCGGGGCCTGCTAATCTGAGGCCGATCCGGGACCGG
  CCTCCTTGCGTCTCCCATTCCCAAGATTGTCCCGCCTCTGCCAATCCCCGCCGTCCTTCCAGCCCACGAC
  CTGCCGCGCCGAGGAGCGGCATCTGTCCCGTTTCCCGATTGGGTCTGTCGTCTCTCTCCGCCTAGCGACA
  GATTCCTTCTATTAAGGGATTGGCTCGCTGAGTTCTAAGCTCTAAATGGGTCAACTCCTTTGTTTTCCGC
  CTAGCGACAAGGGATTTGCTCGCACGGCATTGGCTCCATCCCCTAGTCGCTGGACAGCTCTTTTTTTGAT
  TGGCTCAAATCCTGTAAAGGGCTTGACCAGTCTCTACATAGTCACCGTCCGCTTTTCCTGAGTTCTCCCT
  CCCAATTGGCTCCAGCTTCCTGGGGGCGTGGCCAAGCCCTCCTCTTCCCAGAATTGGCCCGGGGCCTTCA
  ATTTACGTTCTTTACACTACGGGGACTGGGGTCGTCTTTGCCCACGTCCCGACAACTTGTTCCCTGACCC
  CCTCAGGGATGGCCCCAAACTGTCCCTGCCTCTGGCACCCCCTTTCATTGGTTCCATCCATCCCCACAAC
  AGCCTGCCAATCGAAGCCCGTCCCTGCATCCAGGATGGTACCAGCTCCCGCCCCTCGCCCCCCACCTCCA
  CAGGTGCCTTAAAGGGCCCTCGTCCACCCAAGGTGGGGGGCAGGGGCCCTCACTCTCCGGCCCTGGTGTG
  GGGGAGAGAGTGAGGGGTTGGGGGATCGGCAGTTGGGAGGGGCGCTCTGAGATTAAAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_032375.3
• Summary: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]). [supplied by OMIM, Mar 2008]
• Locus ID: 84335