MASA (ENOPH1) (NM_021204) Human 3' UTR Clone

CAT#: SC211607

3`UTR clone of enolase-phosphatase 1 (ENOPH1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ENOPH1
• Synonyms: E1; MASA; MST145; mtnC
• ACCN: NM_021204
• Insert Size: 980
• Sequence Data:
  Insert Sequence

  >SC211607 3'UTR clone of NM_021204
  The sequence shown below is from the reference sequence of NM_021204. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATACCTGCCTTCCTCAACCTAGAGAAGGGTTGTTAAGGCAGACCGCCCTGTTCCCCAGAGTTGTCCCTGT
  AGTGTCTAGGTTTATTCTAATGGTAAAAGTAACTTACTTAAAAAACATATGTACACATATGTATGCAAGT
  ATGTATATATGTGTATGCTCAGATTAACTTCCATAGGTACATAAGTGAAAGAAGTCTCAGTTCAGTGAAC
  ACAAAACTTATTTAAAGATGCTTTATATGTAGAAATTGTTTCAAATCATACTCTAACCCTTAGTGAGGGC
  AAAGTGTAGTTGGTAGAAGAAATTGCTAAATACCTATCTAATGTGCTATGTTTATCAAATCGTGTACTAA
  AATGGAAAGCTAGTTTTGAGAAATTATTCAGAAGCCTTGTTATTTTAAAAATGAAATATTTCAAAGACTG
  AATATTTTCAAAGAAAATGAATAATTCATTGCCCTTGTGATTTAGAAGATTATAACAGCTGTATTTCATA
  TTTGCCTCCTTATATATATCAAAGACCAAGGTATTTCCTTCTGCTTCAAAAGAACAAAATTGGGAAAGAA
  AACTCACTTGAGTCTTGATCAAACAAGTGTCTTTTACTTAAGAAGAAACTTGGTAATCATTGTGGCACCC
  ACAGCAAGCAGTTGCCTTACCAGTGAAAAAGGTGCACTGAGGTAACATCTAAAACAGAGATGTGGTTCTT
  AATGTTTAACAGAACAGTTCTAATCCTGCCACGTGTTATCATTATAGATTTTATAGTTGCCTTTCTAACT
  ACTTAGCACAGTTTGAGAATACGTTAATTGCTATTTACTATTTAAAAAGTTTTACTGAAATCAGTCCATA
  ACATTAAGATGAGCCCTAATATGTAAGATTTTCCTCTGGAATGGATGTGAGAAATGTAAATTTTATAACA
  GCAGTATTTATCCTGGTTTAATTCTAATACGATGTCATGTTAATTTCATGTTGTGATTAATAAAAGCATT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_021204.3
• Summary: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). [UniProtKB/Swiss-Prot Function]
• Locus ID: 58478