KCNJ1 (NM_153767) Human 3' UTR Clone

CAT#: SC212450

3`UTR clone of potassium inwardly-rectifying channel subfamily J member 1 (KCNJ1) transcript variant rom-k5 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: KCNJ1
• Synonyms: KIR1.1; ROMK; ROMK1
• ACCN: NM_153767
• Insert Size: 1106 bp
• Sequence Data:
  Insert Sequence

  >SC212450 3'UTR clone of NM_153767
  The sequence shown below is from the reference sequence of NM_153767. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAAACAGATGACACCAAAATGTAACAGTGGCTTTTCAACGGGAGTAAAGCAAAGTCTCTAAAGCTCCTAG
  TACCTAGAAGCATTATGAAGCAGTCAACAATTTAGGGGTACGAAAGTAGGATGAGAGCCTTCAAAGTCTA
  CCAGCACAAAGACCCCTGAGCCCCGCAATTGTGATCCCACAAGACATGCATCTCCACAAGGCTACTGTAT
  TAGAACGTGCAATGCATTTATATGAAACTGGTGTATGGAAGACATAGGTGCTCTCTTGAAATCTTAAATA
  TGATTATTTGAGCTCATATAAGGTGGATTGGAGCAGATAAAATTATCAAAAGTTTCATGAACAGGCCAAA
  CAAAATATTTTTTAAAGTTTCCTTAAAGAAGTTATGAACTTTAGAAAGGATCAGGGGACAATAATAATCT
  CATTTTGATTCTACTGATAAGAATGACTCCACTTTTAATGTGGACTTTTACTCATGGAAAAATTGTCTCC
  TAATTTGGGGAGATGAACCAACCAATCAATGACAAGAAAACGCTTACACAAAGAACAATTTGAGGCTCTA
  AGCTTCTCATGTGGTACGTTTAGACAGAGGCTAAATCTGCACACTAGAATCTTGATGATACCTTCCTGCA
  AGACAGAATGCTTTAGTTAAAAGTGGTGATGATATTTCTTTCAATCTGTATTGGATGGCTTAAAGGGCTA
  TAAATCTGTTTATAAAGAGCATTTCCTGCTCTTCGAAGACAGCAATGAGGAGTTGGAAGGTGCAAAGTCA
  GTAGAGAAGGGAATGTATCATTAATGCACCTGAGAAGAAACAGTTTCATGTGTTCCTCCACCTAGAGTTT
  GTACTGGAATGCTATTTCTAAAGAAGAAGTGGGAAAGAGAGAGGAATGGGATGGAGCCCCACAGTCAGAA
  TGTTACTATGTCTTTCTTTCCCTGACAGCCCATCTTCCTAAAAGGGACCAGCTTATGGAAGGCTCGACCT
  TGAGGGGAAAGTTTTACTGTGAAAGTCTTCTTCAGATCCCCACCTGCATCATTCCGAATGTGTCCTGGAA
  AAAAACTGGTACTCAAAGCTGCTTAGGAATCAAAATGTTTTCAGTGTGTTGATTAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_153767.2
• Summary: 'Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 3758