Wilms Tumor Protein (WT1) (NM_024426) Human 3' UTR Clone

CAT#: SC213656

3`UTR clone of Wilms tumor 1 (WT1) transcript variant D for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: WT1
• Synonyms: AWT1; GUD; NPHS4; WAGR; WIT-2; WT33
• ACCN: NM_024426
• Insert Size: 1288 bp
• Sequence Data:
  Insert Sequence

  >SC213656 3'UTR clone of NM_024426
  The sequence shown below is from the reference sequence of NM_024426. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CATGACCAAACTCCAGCTGGCGCTTTGAGGGGTCTCCCTCGGGGACCGTTCAGTGTCCCAGGCAGCACAG
  TGTGTGAACTGCTTTCAAGTCTGACTCTCCACTCCTCCTCACTAAAAAGGAAACTTCAGTTGATCTTCTT
  CATCCAACTTCCAAGACAAGATACCGGTGCTTCTGGAAACTACCAGGTGTGCCTGGAAGAGTTGGTCTCT
  GCCCTGCCTACTTTTAGTTGACTCACAGGCCCTGGAGAAGCAGCTAACAATGTCTGGTTAGTTAAAAGCC
  CATTGCCATTTGGTGTGGATTTTCTACTGTAAGAAGAGCCATAGCTGATCATGTCCCCCTGACCCTTCCC
  TTCTTTTTTTATGCTCGTTTTCGCTGGGGATGGAATTATTGTACCATTTTCTATCATGGAATATTTATAG
  GCCAGGGCATGTGTATGTGTCTGCTAATGTAAACTTTGTCATGGTTTCCATTTACTAACAGCAACAGCAA
  GAAATAAATCAGAGAGCAAGGCATCGGGGGTGAATCTTGTCTAACATTCCCGAGGTCAGCCAGGCTGCTA
  ACCTGGAAAGCAGGATGTAGTTCTGCCAGGCAACTTTTAAAGCTCATGCATTTCAAGCAGCTGAAGAAAA
  AATCAGAACTAACCAGTACCTCTGTATAGAAATCTAAAAGAATTTTACCATTCAGTTAATTCAATGTGAA
  CACTGGCACACTGCTCTTAAGAAACTATGAAGATCTGAGATTTTTTTGTGTATGTTTTTGACTCTTTTGA
  GTGGTAATCATATGTGTCTTTATAGATGTACATACCTCCTTGCACAAATGGAGGGGAATTCATTTTCATC
  ACTGGGAGTGTCCTTAGTGTATAAAAACCATGCTGGTATATGGCTTCAAGTTGTAAAAATGAAAGTGACT
  TTAAAAGAAAATAGGGGATGGTCCAGGATCTCCACTGATAAGACTGTTTTTAAGTAACTTAAGGACCTTT
  GGGTCTACAAGTATATGTGAAAAAAATGAGACTTACTGGGTGAGGAAATCCATTGTTTAAAGATGGTCGT
  GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGTGTTGTGTTTTGTTTTTTAAGGGAGGGAATTTATTA
  TTTACCGTTGCTTGAAATTACTGTGTAAATATATGTCTGATAATGATTTGCTCTTTGACAACTAAAATTA
  GGACTGTATAAGTACTAGATGCATCACTGGGTGTTGATCTTACAAGATATTGATGATAACACTTAAAATT
  GTAACCTGCATTTTTCACTTTGCTCTCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_024426.3
• Summary: 'This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]'
• Locus ID: 7490