Optineurin (OPTN) (NM_001008211) Human 3' UTR Clone

CAT#: SC214449

3`UTR clone of optineurin (OPTN) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: OPTN
• Synonyms: ALS12; FIP2; GLC1E; HIP7; HYPL; NRP; TFIIIA-INTP
• ACCN: NM_001008211
• Insert Size: 1444
• Sequence Data:
  Insert Sequence

  >SC214449 3'UTR clone of NM_001008211
  The sequence shown below is from the reference sequence of NM_001008211. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGACATAGACACGTTACAGATTCACGTGATGGATTGCATCATTTAAGTGTTGATGTATCACCTCCCCAA
  AACTGTTGGTAAATGTCAGATTTTTTCCTCCAAGAGTTGTGCTTTTGTGTTATTTGTTTTCACTCAAATA
  TTTTGCCTCATTATTCTTGTTTTAAAAGAAAGAAAACAGGCCGGGCACAGTGGCTCATGCCTGTAATCCC
  AGCACTTTGGGAGGTCGAGGTGGGTGGATCACTTGGGGTCAGGGTTTGAGACCAGCCTGGCCAACATGGC
  GGAACCCTGTCTCTACCAAAATTACAAAAATTAGCCGAGCATGGTGGCGCATGCCTGTAGTCGCAGCTAC
  TCGCGAGGTTGAGGCAGGAGAATTGCTTGAACCCAGGAAGTGGCAGTTGCAGTGAGCCGAGACGACACCA
  CTGCACTCCAGCCTGGGTGACAGAGGGAGACTCTGTCTCGAAAGAAAGAAAGAAAAAAAGGAAGGAAGGA
  GAAGGAAGGAAGGAGAAGAAAAGGTACCTGTTCTACGTAGAACACCTTTGGTGGAGTTCCATCAACTCGC
  AAAGTAGAATCCTTACCTACTACTCTTCTGATAATAATTTTAATATTTTTTATGTTTGGTTGATGCGAGC
  AGCTGCACTGCTCATGCAGTTAGCTAGCATGTGACATCATGTGACAAAGTTCATGTAATTAGATGGAAGA
  AACCTCACTGATTAATTTTAAGAACCTTTTAGGGATGCAGGAACAATGAAGTGGCCACAGTATGTGCTGT
  TTTTGAAGCATTTTTAAAAACGAATTGTAGTTGTTTTTCTTCATTTAAAATGGATCTGTTGGAGGTTATG
  TGTGTATGTTGTAGTTTTATTGCAGCCACAATAATTTTACCAAAGTTTTCACATAGGCAGTTAGCCTTTA
  CTTAATATCAAGACAAGTGAAAAAATATTGGCATCGATGAAACCGATAACATTGGCCTCATTGGATTTCT
  TTACCCATTCACAGTGTAAAGAAGTTACCTTCATGCTTTCATTGTACCTGCAGGCCTGTGGGCTTGTACA
  GTAGATAATTAATTTCTAAAAAGAACAGCTGCCTATTTTCTTCCTAGGTTAGGTTATATCTTCATAATCA
  CAAGAATTAGTGATGGCAAAATAAAATTTTGCTTATGAATCTTTTACATTGTTTATATATGATTAATATC
  ATCATATATATTTTCTGTATTAAGCTCATTTGGCTTCATTTAAGCTGTATACTTAGTCATATATCTTTCA
  TTAGTTCTATGGATATGAGCAGATCCCTTTACTGGAGCCCAGTATGTGCTGTGTGAGTTAGAAGTCATTC
  TTGCTGAGAAGGTGAATAGGTAGGGATTTGCCTTGTTTTGTAAGTCTACAATTTGCCAAGAGTAAATAAC
  ACTGGACCAGCTGTAAAAGTAAACAGTGTGTTTATGCATTGAGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001008211.1
• Summary: This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
• Locus ID: 10133