NQO1 (NM_000903) Human 3' UTR Clone

CAT#: SC215462

3`UTR clone of NAD(P)H dehydrogenase quinone 1 (NQO1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NQO1
• Synonyms: DHQU; DIA4; DTD; NMOR1; NMORI; QR1
• ACCN: NM_000903
• Insert Size: 1618 bp
• Sequence Data:
  Insert Sequence

  >SC215462 3'UTR clone of NM_000903
  The sequence shown below is from the reference sequence of NM_000903. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCAAGTCCATCCCAACTGACAACCAGATCAAAGCTAGAAAATGAGATTCCTTAGCCTGGATTTCCTTCTA
  ACATGTTATCAAATCTGGGTATCTTTCCAGGCTTCCCTGACTTGCTTTAGTTTTTAAGATTTGTGTTTTT
  CTTTTTCCACAAGGAATAAATGAGAGGGAATCGACTGTATTCGTGCATTTTTGGATCATTTTTAACTGAT
  TCTTATGATTACTATCATGGCATATAACCAAAATCCGACTGGGCTCAAGAGGCCACTTAGGGAAAGATGT
  AGAAAGATGCTAGAAAAATGTTCTTTAAAGGCATCTACACAATTTAATTCCTCTTTTTAGGGCTAAAGTT
  TTAGGGTACAGTTTGGCTAGGTATCATTCAACTCTCCAATGTTCTATTAATCACCTCTCTGTAGTTTATG
  GCAGAAGGGAATTGCTCAGAGAAGGAAAAGACTGAATCTACCTGCCCTAAGGGACTTAACTTGTTTGGTA
  GTTAGCCATCTAATGCTTGTTTATGATATTTCTTGCTTTCAATTACAAAGCAGTTACTAATATGCCTAGC
  ACAAGTACCACTCTTGGTCAGCTTTTGTTGTTTATATACAGTACACAGATACCTTGAAAGGAAGAGCTAA
  TAAATCTCTTCTTTGCTGCAGTCATCTACTTTTTTTTTAATTAAAAAAAATTTTTTTTTGAAGCAGTCTT
  GCTCTGTTACCCAGGCTGGAGTGCAGTGGTGTGATCTCGGCTCACTGCAACCTCTGCCTCCCAGGTTCCA
  GCAATTCTCCTGCCTCAGCCTCCCTAGTAGCTGGGATGACAGGCGCCTGCCATCATGCCTGACTAATTTT
  TGTATTTTTAGTAGAGACGGCGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGAT
  CCGCCTACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGATCCACCACACCTGGCCCTTGCAATCTT
  CTACTTTAAGGTTTGCAGAGATAAACCAATAAATCCACACCGTACATCTGCAATATGAATTCAAGAAAGG
  AAATAGTACCTTCAATACTTAAAAATAGTCTTCCACAAAAAATACTTTATTTCTGATCTATACAAATTTT
  CAGAAGGTTATTTTCTTTATCATTGCTAAACTGATGACTTACTATGGGATGGGGTCCAGTCCCATGACCT
  TGGGGTACAATTGTAAACCTAGAGTTTTATCAACTTTGGTGAACAGTTTTGGCATAATAGTCAATTTCTA
  CTTCTGGAAGTCATCTCATTCCACTGTTGGTATTATATAATTCAAGGAGAATATGATAAAACACTGCCCT
  CTTGTGGTGCATTGAAAGAAGAGATGAGAAATGATGAAAAGGTTGCCTGAAAAATGGGAGACAGCCTCTT
  ACTTGCCAAGAAAATGAAGGGATTGGACCGAGCTGGAAAACCTCCTTTACCAGATGCTGACTGGCACTGG
  TGGTTTTTGCTCTCGACAGTATCCACAATAGCTGACGGCTGGGTGTTTCAGTTTGAAAATATTTTGTTGC
  CTTCATCTTCACTGCAATTTTGTGTAAATTTCTCAAAGATCTGAATTAAATAAATAAAATTCATTTCTAC
  AGACCCAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000903.2
• Summary: 'This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]'
• Locus ID: 1728