TEC (NM_003215) Human 3' UTR Clone

CAT#: SC215736

3`UTR clone of tec protein tyrosine kinase (TEC) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TEC
• Synonyms: PSCTK4
• ACCN: NM_003215
• Insert Size: 1656 bp
• Sequence Data:
  Insert Sequence

  >SC215736 3'UTR clone of NM_003215
  The sequence shown below is from the reference sequence of NM_003215. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAATGTGAAGAAACTTTTGGAAGATAAGTGATGTGTGACCAGTGGCTCCCAGATTCCCAAGCACAAGGAA
  GGATGGGCATTTTGTGGCTTTTAATTTATTGAGCACTTGGACATGTAGATCATTTTACTTATACAGTGGA
  AACACATAAATAATTTGCTTCTAGACCAGCCTCTGTCTAGACTTGCTTCTAGACAGAATCTCCCAGAGTG
  TGGAAATGTTGCCTTAGAAATGGTGATTAAAATCACTCATTTCTATTCATTCCTCAGGCACTTGAGTGAC
  AGTTGTTTACCAGGCACTGTGTGTAGCCCCAGGGTTTGGCCATTCAGGGGTGCACACATGGGACCATGTT
  AGCTGATGCCAGTTGAAGGCCAGGGTATTTGGGAAGGGGAAGGGTATTAGAGTCATGACCAAGCAACCCT
  TCTTTTTCCCTTTGACTTCTACAGAAATCTGGGCCTGAGACATTGTCTACAATTGGGTTCTAGATACATC
  AGGAACCCATCTTGGATAAATAAATACCTATCTTTTGTTTTGAAAACATCTCAGTTTTCAAGACTGCTCT
  TAGTATTACATGAACAATATTTGTATGCTGTATATATTGTAAATATATATAATATATAAAGTTATATATT
  TATGAGAAACACGAATTGTCTTTTAATTGAAACTTTTAATCCTGTAGTATAGGAGTTCACCTTCTTAGGA
  CTAGAGACTGTGCCTTATAGCTGTTAATTCATTTCCCCCTGAACATCAAATATGCCTGAAGAGAAGAAAG
  TCTAGATTCTTCTATGAGTAACGCCCCCTCCTCACTCAGGTAAATGTGTCTGGGGATGCCTGTCCAGCTT
  AACCACGTGCATTTGGCCTATGTAATCCTGCCCATGGTGGCCGCAGCTAATCAGAATCAGATGGAAAATT
  AAACCGGGTAATCTACTTCTAAGCCTTAAGAATATTCCCTGGGACACAGACACTATAATTGGAAGTGCTG
  AGCTCTGGGGCAGAAGGATCAGGTGACCTTCGCAACAAAGTTTGCCCCCACCTCACATAGGACCCGGAAG
  CAGCCTGAGCTGTGGCGGAGGATCCAGGAAGCTACGGAGAGAAGCAGCCAGCATGGTGTTCCGTGCCTCC
  CGGACGTTTTTCAGGAGGCCTGGTTGGACTTGGGTTCCTGGATGGTGGGATTGTTGTACAGCCTCTCAGG
  AGACCCTGCTGTCAAGACTGTGTGTGTGGATTTCTCACCCTTAGAAGCTCTACTAAGACATCAACGGAAT
  TAGGGCCTTCCTTTTTGCCTTGTGAGCGCCAAGGAAAAGAAACTATCTCGGTCACGTGAGCGCCAGCGAA
  AAGAAACTGTATCAGTCATCCAGAGACCGTTTATTGCCCAACACGTTATTCTTGCTGTTGGTGGGGTAAC
  TAGCCGAGGAAGACACAGCGCCTTCCCTTCAGGAGTTGCGTCTCCTCTGCAGGCCACGATGGTCTGCTCT
  GGAGCATTGGGTGAACACACAGGCTGGCTGCTCTGGGCAGCGCCTTCACTCTGACCCTGGAGAACCATTT
  CATTTCATCCTGGTCAGTCTAGAGTCTGTGCACCAGGCAGTCCATCCACTGAAGGCTGTGTTTATTCTTT
  TCCTGTGCCCCTCATAATGGAAGAAAGTAAACTGCTTATCCCGAGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003215.2
• Summary: 'The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome. [provided by RefSeq, Jul 2008]'
• Locus ID: 7006