TBX5 (NM_000192) Human 3' UTR Clone

CAT#: SC216065

3`UTR clone of T-box 5 (TBX5) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TBX5
• Synonyms: HOS
• ACCN: NM_000192
• Insert Size: 1616 bp
• Sequence Data:
  Insert Sequence

  >SC216065 3'UTR clone of NM_000192
  The sequence shown below is from the reference sequence of NM_000192. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAGAGTGGAGCGACAATAGCTAAAGTGAGGCCTGCTTCACAACAGACATTTCCTAGAGAAAGAGAGAGA
  GAGAGGAGAAAGAGAGAGAAGGAGAGAGACAGTAGCCAAGAGAACCCCACGGACAAGATTTTTCATTTCA
  CCCAATGTTCACATCTGCACTCAAGGTCGCTGGATGCTGATCTAATCAGTAGCTTGAAACCACAATTTTA
  AAAATGTGACTTTCTTGTTTTGTCTCAAAACTTAAAAAAACAAACACAAAAAGATGAGTCCCACCCCCCA
  CTACCACCACACCCATCAACCAGCCACATTCACGCTACTCCCCAGATCTCTTCCCCCATTCCTTCTTTTG
  GGCTCTAGAAAGTCTTGCCTCATTGAGTGTTTTTCCCTAGTGCGTAGTTGGAGTCTGTCCCTGTCTTGGT
  GTTAATGTTGACATTGTTATATAATAAATGATAATATATTTTTTTCTTTCAATTTTCTTAATGGGACCCA
  GTCCCTTATTTGGGGGGAGGTCTGAGGCAAGTATATTTCAAAATATGTACTTGCGGGATTCCCTTCAAGT
  AAACCATCCCTGAAACCTAAATTCACGTTTCCCCTTGACTAAGAAAAGCACCTACCTCTGCCATGTGATG
  TTTCTGAAAAGCCTCTGTATGTCCCCATTTGCTTTGGTTTTGTCCTGCCTTCTCCAATATCACGTGCTCA
  GTTTTGCCTCTACTTACCCATGGAGTCAGGATAACACTGACGCTCCCTGGCATCCTATCTTATTCAGCCC
  TACCATCTTGCCAGCTCTGTCTTTCCAGCTGTCTGTCGCTAAAACGTGGCCTATAGCTTCCCTTCCGGAA
  AGCTTGCTTTGAAAAACTTAAAAAGCCCCCGTTTACATGTAGGCAGGACTGTGATAACAGTGCAAGCTCT
  GTGTTGACAAGAGTTGTGGACAAAAAGCCAAAATAAATATTCTTCCTGATTAAAAAAATTTTTTTTGAAA
  AAAACAAGGCCAGCCCCAACCTTCCAAACCTCCATCACCAACAACCCAAACTGGATGTCAAGCAAAATGC
  ACAATTCCTACAGAAGAGGCAAGACACAGTCACCAATGATATCTCGCCAAAGAAACCACGCCCACACCAA
  TGCCAACACAAAACTGTGTTTACTGAAAGCCGAAAACAGTATTAAAAAAAGTGTGTAAGTAAAGTGTTAT
  GGTAGGGTTCTTCAGATGTAATATTTTACTGGTACTATTTATTTATAAATAGGAATTCTAATTAAGTAAT
  AACATGAAATGAAACCCAGCATAGGAGCTGGCCAAGAGCTTTTAATTTTATTGATACTCAAAACCAAGTT
  TGTGTTTTTTTGTTTTTTTTTGTTTTTTTCCTCTTTCGAATGTGCTTTGCTTTTTTTGATTAAAAAGAAT
  TTTTTTTTTCCTTTTTTATAAACAGACCCTAATAAAGAGAACAGGGTAAGATGTGAGGCTGAGTGTGTTT
  AAGTACGTGAGAGAGTGTGAGTGTGTTTGTAAGTGAGTGTCCCTATGCGATTATGTCTCTTTACGTTGCT
  AAGGGGGGAGGGTGAGGATTAAGTACTCGTGCCTTATATTTGTGTGCCAATTAATGCCTAATAAATACCA
  TGTGCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000192.3
• Summary: 'This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 6910