PCBP2 (NM_005016) Human 3' UTR Clone

CAT#: SC216227

3`UTR clone of poly(rC) binding protein 2 (PCBP2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PCBP2
• Synonyms: hnRNP-E2; HNRNPE2; HNRPE2
• ACCN: NM_005016
• Insert Size: 1765 bp
• Sequence Data:
  Insert Sequence

  >SC216227 3'UTR clone of NM_005016
  The sequence shown below is from the reference sequence of NM_005016. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAATGTCAGGCTTTCCTCGGAGACGGGTGGCATGGGGAGCAGCTAGAACAATGCAGATTCATCCATAATC
  CCTTTCTGCTGTTCACCACCACCCATGATCCATCTGTGTAGTTTCTGAACAGTCAGCGATTCCAGGTTTT
  AAATAGTTTGTAAATTTTCAGTTTCTACACACTTTATCATCCACTCGTGATTTTTTAATTAAAGCGTTTT
  AATTCCTTTCTCTGTTCAGCTGTTGATGCTGAGATCCATATTTAGTTTTATAAGCTTCTCCCTGGTTTTT
  TTTTTTTGGCTCATGAATTTTTCTGTTTGTCATGGAAATGTAAGAGTGGAATATTAATACATTTCAGTTT
  AGTTCTGTAATGTCAGGAATTTTTCAAAAAAATTAAAAGATGGACTGGAGCTTTTTCTTTGTGAATAGAA
  ACTGGATGCCACAGTGATTCATGTGGGTTTTATTCCTCTTGTCTTGCTGTTATTTTTGTACCTTTTATCC
  CTCAAAGGACCCTTCTTGGGTTTTGAATGGAAGCCTTTATTCCGGTTAAGATGTTTTCTTCTATTTTACC
  ACTTCCATCTTTTTTTGTGGCCCTCGATCCTATTTTTCCCTGACTCCATGCTTGGTTGGCCCTTATAAAA
  CTTGTGCCCAAAAGATTGAGGATTAGACTTTCCGAGGACTTACCTGTCCTAGGGGAGTAGGCAAGCACTT
  CCACTAGGGAGGGGGTGGGGGAAAGGAATGACACATGACATACATGGCATACACATTAAGCAGTTGATCA
  TATGTCTGACTGGGTTCCAGTTTCTTGGGAATGTTGGTCCCCTTGTTCAGGCTTGCATATTTTAAACTAA
  AAATTTCAGTCTATTGTTTTTAGTAACTTCATTTATAGTCCTCCATAACAAGTTAGAAGGATGTATCTGC
  TACCATTTATTCCTATAATTTTAGAAAGTTGGGGCTTGACATTATACTCATTTAGTGAGAGTAGATGCAA
  AAAAGTGGAGGGGCAGGAGAACTTCTCCAGACACCTCAGATAAAGTCCGGAGCCCAAGGCTTTATCTTAA
  CCATGTATGGTACCCCATTCATTCATCAAGAAAACCCTCAACAGCTGGGCCTGCATGGAGTGTTATATTT
  CAAGGTTTTTCACAGGGGTTACAGTAGGACAGTCCCCACCCCAATCAGGCACCAGGATAAAAGCAGGGAC
  TTAAACAGCACCCCGGTTCTTCAGCCTGAGCCATCACATGCTATCAGTCTCCTAACCTCCCCCTGGGCCT
  TAAGACAGGGCTTGGGCAGAGAAGATAAATGGTGGGACAAAAAAATGAGTTACATTGCCACCTGAGAAAC
  CTCAGAGGGGAGGACCCAGCCTTAGCCTCCCTCCTCCCAAGTGCAAAATGTGTAAACAGAGTAAACGGAA
  CAGAAAAGTGCAGTCTAAGTGGTTTTCTCTCCTGCCCCTCCCACCGCCCCTCCCCCCACCCCCTATTATT
  TGGGGATAAAGAATATAAAGACAACCCTGGCTTTTCTATTGCCTTGTTGCTTGCTGAATATAAGGAATGG
  GGTGGGGCAGGAAGGGGCTTGCCCTTAGCCACAGCTCTACGGCTGTGCCTCATTCATTTCCACAGCTGCC
  AGTGTCCCTAGAGTTTATCAGGTGAATTGGTCAGGGGATCAGTCTCCCTCGAGCCTGACTTACGGCTGGG
  ACAGCCCCATCTTTCTGTTGATTATGTGGCGCATATATATATATATATGTATATATATATAATTTATATA
  AATATTTCTCTATGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005016.5
• Summary: 'The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2018]'
• Locus ID: 5094