PCBP2 (NM_001128912) Human 3' UTR Clone

CAT#: SC216229

3`UTR clone of poly(rC) binding protein 2 (PCBP2) transcript variant 5 for miRNA target validation

Reconstitution Protocol

USD 670.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol PCBP2
Synonyms hnRNP-E2; HNRNPE2; HNRPE2
ACCN NM_001128912
Insert Size 1765 bp
Sequence Data
>SC216229 3'UTR clone of NM_001128912
The sequence shown below is from the reference sequence of NM_001128912. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

CAATGTCAGGCTTTCCTCGGAGACGGGTGGCATGGGGAGCAGCTAGAACAATGCAGATTCATCCATAATC
CCTTTCTGCTGTTCACCACCACCCATGATCCATCTGTGTAGTTTCTGAACAGTCAGCGATTCCAGGTTTT
AAATAGTTTGTAAATTTTCAGTTTCTACACACTTTATCATCCACTCGTGATTTTTTAATTAAAGCGTTTT
AATTCCTTTCTCTGTTCAGCTGTTGATGCTGAGATCCATATTTAGTTTTATAAGCTTCTCCCTGGTTTTT
TTTTTTTGGCTCATGAATTTTTCTGTTTGTCATGGAAATGTAAGAGTGGAATATTAATACATTTCAGTTT
AGTTCTGTAATGTCAGGAATTTTTCAAAAAAATTAAAAGATGGACTGGAGCTTTTTCTTTGTGAATAGAA
ACTGGATGCCACAGTGATTCATGTGGGTTTTATTCCTCTTGTCTTGCTGTTATTTTTGTACCTTTTATCC
CTCAAAGGACCCTTCTTGGGTTTTGAATGGAAGCCTTTATTCCGGTTAAGATGTTTTCTTCTATTTTACC
ACTTCCATCTTTTTTTGTGGCCCTCGATCCTATTTTTCCCTGACTCCATGCTTGGTTGGCCCTTATAAAA
CTTGTGCCCAAAAGATTGAGGATTAGACTTTCCGAGGACTTACCTGTCCTAGGGGAGTAGGCAAGCACTT
CCACTAGGGAGGGGGTGGGGGAAAGGAATGACACATGACATACATGGCATACACATTAAGCAGTTGATCA
TATGTCTGACTGGGTTCCAGTTTCTTGGGAATGTTGGTCCCCTTGTTCAGGCTTGCATATTTTAAACTAA
AAATTTCAGTCTATTGTTTTTAGTAACTTCATTTATAGTCCTCCATAACAAGTTAGAAGGATGTATCTGC
TACCATTTATTCCTATAATTTTAGAAAGTTGGGGCTTGACATTATACTCATTTAGTGAGAGTAGATGCAA
AAAAGTGGAGGGGCAGGAGAACTTCTCCAGACACCTCAGATAAAGTCCGGAGCCCAAGGCTTTATCTTAA
CCATGTATGGTACCCCATTCATTCATCAAGAAAACCCTCAACAGCTGGGCCTGCATGGAGTGTTATATTT
CAAGGTTTTTCACAGGGGTTACAGTAGGACAGTCCCCACCCCAATCAGGCACCAGGATAAAAGCAGGGAC
TTAAACAGCACCCCGGTTCTTCAGCCTGAGCCATCACATGCTATCAGTCTCCTAACCTCCCCCTGGGCCT
TAAGACAGGGCTTGGGCAGAGAAGATAAATGGTGGGACAAAAAAATGAGTTACATTGCCACCTGAGAAAC
CTCAGAGGGGAGGACCCAGCCTTAGCCTCCCTCCTCCCAAGTGCAAAATGTGTAAACAGAGTAAACGGAA
CAGAAAAGTGCAGTCTAAGTGGTTTTCTCTCCTGCCCCTCCCACCGCCCCTCCCCCCACCCCCTATTATT
TGGGGATAAAGAATATAAAGACAACCCTGGCTTTTCTATTGCCTTGTTGCTTGCTGAATATAAGGAATGG
GGTGGGGCAGGAAGGGGCTTGCCCTTAGCCACAGCTCTACGGCTGTGCCTCATTCATTTCCACAGCTGCC
AGTGTCCCTAGAGTTTATCAGGTGAATTGGTCAGGGGATCAGTCTCCCTCGAGCCTGACTTACGGCTGGG
ACAGCCCCATCTTTCTGTTGATTATGTGGCGCATATATATATATATATGTATATATATATAATTTATATA
AATATTTCTCTATGT

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_001128912.1
Summary 'The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2018]'
Locus ID 5094

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.