POLK (NM_016218) Human 3' UTR Clone

CAT#: SC216518

3`UTR clone of polymerase (DNA directed) kappa (POLK) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POLK
• Synonyms: DINB1; DINP; POLQ
• ACCN: NM_016218
• Insert Size: 1828
• Sequence Data:
  Insert Sequence

  >SC216518 3'UTR clone of NM_016218
  The sequence shown below is from the reference sequence of NM_016218. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAATCCCAAACATACCCTTGATATATTTTTTAAGTAAACATTGAACATTTTATCATTAATTTTTAATTGA
  AACTAGTTATTTTATAATCAATGAATTTGTTCTTTCTGATTTTAAGTTTGCAGATTTATTTAGTGAAGGC
  AAGTGCAATAATCCTTCCTCAGATGATGTTTGCTTTTCTAAGATACATATACTGATTCTGTGTATCTTTT
  TTATAACCATGAGAATTTTACTTCCATTATACATCAATTGGAAATCAATCCTGTTAAGAGATAATTCTTA
  AAAGGGAAATTAGGAATGGGATAAGAAGGTGATTTTTTTATTATTTTTATACTGAATATAAAAACATTTG
  TAAGGGCTCTCAAAGATTCACACATGCCTATATTATCATAAGAATTTTTCAGCACTTAACTACTTTGTTG
  GCATTGATCCTAGTGTCTTTAAATACTTCATGAGCATTCATAATTAAAATTTATCTTAAGTTCTATGAAG
  AGTATTAATGTAACTAGCATAAGTGGTTTTCTTCAGGAAAATAAATATCACAGTATTATCTGTGTTAAAA
  TGGTTTTTGCCTAAAATATAATTTTTAATTTGGCTTTTCTTATTTAAAATTCCATTATCTTATGAATAAG
  CACTTGAATCAGTTTTTAAAATATTTAGTCTAAGATGATTCAAAGTAGTTTTATTTTAATACAGGACTTT
  TAAATGGCAGTATTTCATTTCTTGTCAATTATGTTGGTACTTTCCACAAATCTATAAAGAAGGATAAATT
  GTACCATCATTTTATTATAATCCTCAAGAGAAAATGTGTAATTCAAAAGATTAATGTGTATTAAAACACA
  TTATGTATCTTAGTTACATTTCTATCAGTACTTTTATTAATATTTGTGAAAGAAGACAGCTTAATAGTAG
  TTAGCTTAAGTAGTTTCTCCAAGTACTTTTGTGCTATCAATGAGTTCTTCTCAAAAAATAATTAGTTAGG
  CCAGGCACAATGGCTCACACCTGTAATGCCAGCCCTTTGGGAGGCCGAATGGGCAGATCACTTGAGGTCA
  GGAGTTTGAGACCAGCCTCGCCAACATGGTGAGACCCTGTCTCTACTAAAACGATAAAAAAAAAAAAAAA
  ATTAGCCAGGCTTGGTGGCACACGCCTGTAATCCCAGCTACTCAGATGGCTGAGGCAGGAGAACTACTTG
  AACCTGGGAGGTCAAAGCTGCAGTCAGCCAAGATCTTGCCACTGTACTCCAGCCTGAAGAGCGAGACTCT
  GTCTCAATAATATAATAATAGTTATTATTTAATTGCAACATGAAGTTGGAAGCCATTTTCTGTTACTTCT
  TTAAGGAGCTAAATCTTTTATATTTTTGTTTTCTCTCCCCTTGCGTTTCATATTTTAGCTTATGATCTAA
  ATATATATTTTGTATATTATAATATTTTAGGAATAGCTTAATTAAGAACTCACAGACTTTGACAAAGTGG
  TAACTAGACTTTTAATTACTAGATTCTTCAAATTAGGCTTAATGCCTGCTAGCATGCCAATCCAATCCCA
  TGACTGATGTTATTATACACTATGGCAAATACAAAGTTGTAGTATTATGGTTCTTGATGGTGTGATTTAC
  TTATGTAAATTATGTACAAATTTAAGTTCAATGAAACACTGATTTAATGTACTGAAAGGTAAATAGTACC
  ATAGTGAATTATTTTCTGTCTTGCCTTAGGTTTATTTTTATTATCTTCAATATTAGAATAAATGCTAAAT
  TATTTTCATTTAAGGATTTATGTAGTTGAATATTATCATTTAGTAATATTTAATCATTTGAAATAAAATT
  TAAAGCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_016218.2
• Summary: This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016]
• Locus ID: 51426