Factor VIII (F8) (NM_000132) Human 3' UTR Clone

CAT#: SC216618

3`UTR clone of coagulation factor VIII procoagulant component (F8) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: F8
• Synonyms: AHF; DXS1253E; F8B; F8C; FVIII; HEMA
• ACCN: NM_000132
• Insert Size: 1799 bp
• Sequence Data:
  Insert Sequence

  >SC216618 3'UTR clone of NM_000132
  The sequence shown below is from the reference sequence of NM_000132. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGCACAGGACCTCTACTGAGGGTGGCCACTGCAGCACCTGCCACTGCCGTCACCTCTCCCTCCTCAGCT
  CCAGGGCAGTGTCCCTCCCTGGCTTGCCTTCTACCTTTGTGCTAAATCCTAGCAGACACTGCCTTGAAGC
  CTCCTGAATTAACTATCATCAGTCCTGCATTTCTTTGGTGGGGGGCCAGGAGGGTGCATCCAATTTAACT
  TAACTCTTACCTATTTTCTGCAGCTGCTCCCAGATTACTCCTTCCTTCCAATATAACTAGGCAAAAAGAA
  GTGAGGAGAAACCTGCATGAAAGCATTCTTCCCTGAAAAGTTAGGCCTCTCAGAGTCACCACTTCCTCTG
  TTGTAGAAAAACTATGTGATGAAACTTTGAAAAAGATATTTATGATGTTAACATTTCAGGTTAAGCCTCA
  TACGTTTAAAATAAAACTCTCAGTTGTTTATTATCCTGATCAAGCATGGAACAAAGCATGTTTCAGGATC
  AGATCAATACAATCTTGGAGTCAAAAGGCAAATCATTTGGACAATCTGCAAAATGGAGAGAATACAATAA
  CTACTACAGTAAAGTCTGTTTCTGCTTCCTTACACATAGATATAATTATGTTATTTAGTCATTATGAGGG
  GCACATTCTTATCTCCAAAACTAGCATTCTTAAACTGAGAATTATAGATGGGGTTCAAGAATCCCTAAGT
  CCCCTGAAATTATATAAGGCATTCTGTATAAATGCAAATGTGCATTTTTCTGACGAGTGTCCATAGATAT
  AAAGCCATTTGGTCTTAATTCTGACCAATAAAAAAATAAGTCAGGAGGATGCAATTGTTGAAAGCTTTGA
  AATAAAATAACAATGTCTTCTTGAAATTTGTGATGGCCAAGAAAGAAAATGATGATGACATTAGGCTTCT
  AAAGGACATACATTTAATATTTCTGTGGAAATATGAGGAAAATCCATGGTTATCTGAGATAGGAGATACA
  AACTTTGTAATTCTAATAATGCACTCAGTTTACTCTCTCCCTCTACTAATTTCCTGCTGAAAATAACACA
  ACAAAAATGTAACAGGGGAAATTATATACCGTGACTGAAAACTAGAGTCCTACTTACATAGTTGAAATAT
  CAAGGAGGTCAGAAGAAAATTGGACTGGTGAAAACAGAAAAAACACTCCAGTCTGCCATATCACCACACA
  ATAGGATCCCCCTTCTTGCCCTCCACCCCCATAAGATTGTGAAGGGTTTACTGCTCCTTCCATCTGCCTG
  ACCCCTTCACTATGACTACACAGAATCTCCTGATAGTAAAGGGGGCTGGAGGCAAGGATAAGTTATAGAG
  CAGTTGGAGGAAGCATCCAAAGATTGCAACCCAGGGCAAATGGAAAACAGGAGATCCTAATATGAAAGAA
  AAATGGATCCCAATCTGAGAAAAGGCAAAAGAATGGCTACTTTTTTCTATGCTGGAGTATTTTCTAATAA
  TCCTGCTTGACCCTTATCTGACCTCTTTGGAAACTATAACATAGCTGTCACAGTATAGTCACAATCCACA
  AATGATGCAGGTGCAAATGGTTTATAGCCCTGTGAAGTTCTTAAAGTTTAGAGGCTAACTTACAGAAATG
  AATAAGTTGTTTTGTTTTATAGCCCGGTAGAGGAGTTAACCCCAAAGGTGATATGGTTTTATTTCCTGTT
  ATGTTTAACTTGATAATCTTATTTTGGCATTCTTTTCCCATTGACTATATACATCTCTATTTCTCAAATG
  TTCATGGAACTAGCTCTTTTATTTTCCTGCTGGTTTCTTCAGTAATGAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000132.3
• Summary: 'This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]'
• Locus ID: 2157