SYBL1 (VAMP7) (NM_001145149) Human 3' UTR Clone

CAT#: SC216625

3`UTR clone of vesicle-associated membrane protein 7 (VAMP7) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: VAMP7
• Synonyms: SYBL1; TI-VAMP; TIVAMP; VAMP-7
• ACCN: NM_001145149
• Insert Size: 1836 bp
• Sequence Data:
  Insert Sequence

  >SC216625 3'UTR clone of NM_001145149
  The sequence shown below is from the reference sequence of NM_001145149. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCTCTCTGTGGTGGATTTACATGGCCAAGCTGTGTGAAGAAATAGGAAAGAAGAAGTTACCATTAACCAA
  GGATATGAGAGAACAAGGAGTTAAAAGCAATCCATGTGACTCAAGCCTTTCACATACTGACAGATGGTAT
  CTGCCAGTCTCTTCAACCCTCTTCTCACTTTTTAAAATCTTGTTCCATGCCTCCAGGTTTATCTTTGTCT
  TATCTACCAGTTTATTCCTGTGAACTTCAGATTGAACCATTCATTGCAGCAGTAGCCTTAAAAAGGCTTT
  TGTTTATTTCTTTGGTTTGTTAACTAGTGTCATCTATTTAGAGAAACATTTTTGTTTTTAATTGCTCAAA
  GCTGTCGCCGCTAGTCTTATGAGCTATCTACTAAAACTATGGAGAAACTTTGTATGTGCACACAAAAGTA
  TTCAAGAGACAGTATTGCTAACATCTCATCTTAATGTCTTTTGTTATTGAGAAGTTTTAGGTGCTTCAAA
  ACAATATAAATGGATAATAGTTGTTATTTGGGGAATTGTAATGATGTTGGTGCTGCTTCCTTCTAAGAGC
  TCAGACAAGTAAAGTATGAAACATTCTTATTTCAGTTAGATGGGGAACATTTTGCTAGCCCATTAGAAGC
  ACACAGAATTATCCTTGTCCTCCTAATATTGACTTTCAGGAATAAAGTTCAGTGTGCTGATCATTCACAA
  TACAGTGGATAGCTTGATATCTTCTGTTTTCCCATTGCAGTTGATTTGAGAAGATGAAGGTTTAAATATT
  GTTGAAAGTTGCAGTTTTTTAAATGTGTTCCTTTTTCTTCTGTGAATATTTAGGGCAATCGTGTCGCTAA
  TAGAATATGTAGTAGAGGGGGTGGGGAGGTAAATTCCTCTGACTTGCCAAAGAAAAAGAAGGGAACCACA
  GTGGATATGCTAGCATTTTAGCTGTGCAAAGGGAGGTAGTGTGGGAAAAGTGTTTCCATTCTGGGAAAAG
  CCCAAACCGAATACGGTCAGCAGTCAACTCCAGGGTTTGGGCTTGATTCCTGTTGAATAATAGTTTTGAG
  CATTCTTTGTGGTTAAATAAATTCTTAAATCTGCCTAGTTTTGATGAATTCTTTTGTGAAACTTGAAAGA
  GAATAGACAGTATGACATATAGAATTAATACAAAACAGTTTAACAACCATTTAACTGCAGTGTAAGAAAA
  TTGGACTGTAATCATATCGCTACTGGCATCTGTTATCTAGTATGCATTTCTGGTGTGTATCTGAAAGGAA
  GACATTTTCTACCCTAGATCCAATTGCATTTATTTATCAATAAGTGCCATTAAATTGAAATTATATTACA
  TTTTACACTTTCTCAATGAATGAACAAATTAGTCTGTAGAATCTAGCCACCTGTTTAGCCTAGTCATGTG
  CCTTGAACATATATGTGTCCCATAATCTGGCTCATGGTACCTGTTCTTCTATCCAAACCTTTCAATTCAT
  GCTACCTGATTCATTTATTTGACATAGATCTTAGGCCCACTTGAACTCTTTTCTTGTTTATCTAGCATAG
  CACAAACGTTTTTCCAGTCTTCTTTATCAACACTAATGCCTCTTAATTGCATCAGTATTTCCTATTGGAA
  AATACATCTGTTCCAGAAAAACATTTGGCATTCCTGAATAATTTCCAAATGTTTTTAATCCAAAGAAAAA
  GGTTTAAAGCTTATTTCCCTTTCTTATACACACCTGAATAAAATTGATGTGCATGTTTTAGGGATCAATT
  ACCTAACTGTTCCTTGGTCTATTTATGTATAAGAATGCTTTTTAAAGCACATGTCTCATTTTAAATGACG
  CACAAACTGAAGATGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001145149.1
• Summary: 'This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]'
• Locus ID: 6845