Glypican 4 (GPC4) (NM_001448) Human 3' UTR Clone

CAT#: SC216672

3`UTR clone of glypican 4 (GPC4) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GPC4
• Synonyms: K-glypican
• ACCN: NM_001448
• Insert Size: 1868 bp
• Sequence Data:
  Insert Sequence

  >SC216672 3'UTR clone of NM_001448
  The sequence shown below is from the reference sequence of NM_001448. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTTCTGCATCTTGTTCCTGGTTATGCAGAGAGAGTGGAGATAATTCTCAAACTCTGAGAAAAAGTGTTCA
  TCAAAAAGTTAAAAGGCACCAGTTATCACTTTTCTACCATCCTAGTGACTTTGCTTTTTAAATGAATGGA
  CAACAATGTACAGTTTTTACTATGTGGCCACTGGTTTAAGAAGTGCTGACTTTGTTTTCTCATTCAGTTT
  TGGGAGGAAAAGGGACTGTGCATTGAGTTGGTTCCTGCTCCCCCAAACCATGTTAAACGTGGCTAACAGT
  GTAGGTACAGAACTATAGTTAGTTGTGCATTTGTGATTTTATCACTCTATTATTTGTTTGTATGTTTTTT
  TCTCATTTCGTTTGTGGGTTTTTTTTTCCAACTGTGATCTCGCCTTGTTTCTTACAAGCAAACCAGGGTC
  CCTTCTTGGCACGTAACATGTACGTATTTCTGAAATATTAAATAGCTGTACAGAAGCAGGTTTTATTTAT
  CATGTTATCTTATTAAAAGAAAAAGCCCAAAAAGCAGTAAAATTTCCATTTCTCCCTGTTATTTTAGTTG
  CCTTATCTGGAGAGACGTGGAGGTGATTTTCTTTTTTTTAAATTATTATTAAGACAGAATGTGAGGGCAC
  AAGCAGGCTTCTGAGCCACTTGTCAGATTGTATTCAAAGCATCAATCCAAGAAGGAGGTTATGTGTACTT
  CATTTATTGGTGATAGTTGGAAGAGACTGCAGACTACTGCTTTGAATGAGTTGAATTACATAAGCTAAGA
  TCACTATAGGTCCATTTCTTGAACCCACTTATACATAAAATGTAACCCATTTAGAAAAAGATTCTGGATA
  TCATCCCCCTTGAAAGATAGAAAGCATTCAGGATGTCCCAGTTATCACATGTTCACACTTGGGTTTAGGG
  GTGTTTTTTTTTAAAACCAGGCAGGTTAGCTAGCCCACCCTGTGCTAGTTTTCATGTTCACACTGACCCT
  ATTTGAATTAATATCCTTTGTTAGAGTGGTCGAGATTTCAAACCCAATTATGTACAGGGAGCTGTCTGAG
  AGCTAGCCAGAACTGGGGTACAGCCTGGGCTCAGGGAATAGCTGTCAACACTCGGGCAAAGTTTTTGTCT
  GTGCATGTGTATCTCCATTTGTTTTGGGATCCCAGTTTTTGTTTTAAGAGAGTATAAGGTGTCTCATTTG
  AGTCTTTTTCTTACCTAGCCCCCTCTTATCAGTAAAACAAAGGACTTGCCATGGTTCACAGCAATGTGCT
  ACGATCCAAGATATCAGCCAAGGAGCCCACTTAGGGGAGAACTAGGTGTCCAGATTTTTGTATGTGTTGT
  TTTTCTTGGGGGATGGGGTGGGGTGGGAGTAGGTAGAGCTGAGAATACTACATCTTAGTGGTGACCTTTA
  GCCACGTGGGTGAAGTGGCAAAGGCCATGGCCATATCTGTTGTCCCAGGCCAAAGACTAACAACTGCCTT
  GGGAATCCCTTCCTTGTGTCCTTACCAAATGATAGCTCATAAAACTCTGATAATGTAACAAATCACTTTC
  AAAGGAGTTCCCAGAAGTCTTCAGAAAGACTAAAATTCTGTCTCTTCCTGCTTTAGACAGCCATTAAGAT
  CCCAACTAATTTTACCGAACCTAAAACCCACAAAGAGGTTGTTTGTGTTATTGTTCAATCTTCAGTTGTA
  AGAGTAATTCTCTATTTTTATATTGAAACATAATTACTTGATAGCTCAGGGTCTACATTTCATTCAACTT
  TTTACACCAAATTCTGCAGAGTGGTCAAAATGGAATATTGGGGGCTGTTGTAAACAGAGGCTTAATTTTA
  TTAGAAGTAGCCAGTTATTTATTAAAGCATGATGTTAATAAAATAGGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001448.2
• Summary: 'Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq, Jul 2008]'
• Locus ID: 2239