BMI1 (NM_005180) Human 3' UTR Clone

CAT#: SC217171

3`UTR clone of BMI1 polycomb ring finger oncogene (BMI1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: BMI1
• Synonyms: flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51
• ACCN: NM_005180
• Insert Size: 1967 bp
• Sequence Data:
  Insert Sequence

  >SC217171 3'UTR clone of NM_005180
  The sequence shown below is from the reference sequence of NM_005180. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGGTCATCAGCAACTTCTTCTGGTTGATACCTGAGACTGTTAAGGAAAAAAATTTTAAACCCCTGATTTA
  TATAGATATCTTCATGCCATTACAGCTTTCTAGATGCTAATACATGTGACTATCGTCCAATTTGCTTTCT
  TTTGTAGTGACATTAAATTTGGCTATAAAAGATGGACTACATGTGATACTCCTATGGACGTTAATTGAAA
  AGAAAGATTGTTGTTATAAAGAATTGGTTTCTTGGAAAGCAGGCAAGACTTTTTCTCTGTGTTAGGAAAG
  ATGGGAAATGGTTTCTGTAACCATTGTTTGGATTTGGAAGTACTCTGCAGTGGACATAAGCATTGGGCCA
  TAGTTTGTTAATCTCAACTAACGCCTACATTACATTCTCCTTGATCGTTCTTGTTATTACGCTGTTTTGT
  GAACCTGTAGAAAACAAGTGCTTTTTATCTTGAAATTCAACCAACGGAAAGAATATGCATAGAATAATGC
  ATTCTATGTAGCCATGTCACTGTGAATAACGATTTCTTGCATATTTAGCCATTTTGATTCCTGTTTGATT
  TATACTTCTCTGTTGCTACGCAAAACCGATCAAAGAAAAGTGAACTTCAGTTTTACAATCTGTATGCCTA
  AAAGCGGGTACTACCGTTTATTTTACTGACTTGTTTAAATGATTCGCTTTTGTAAGAATCAGATGGCATT
  ATGCTTGTTGTACAATGCCATATTGGTATATGACATAACAGGAAACAGTATTGTATGATATATTTATAAA
  TGCTATAAAGAAATATTGTGTTTCATGCATTCAGAAATGATTGTTAAAATTCTCCCAACTGGTTCGACCT
  TTGCAGATACCCATAACCTATGTTGAGCCTTGCTTACCAGCAAAGAATATTTTTAATGTGGATATCTAAT
  TCTAAAGTCTGTTCCATTAGAAGCAATTGGCACATCTTTCTATACTTTATATACTTTTCTCCAGTAATAC
  ATGTTTACTTTAAAGATTGTTGCAGTGAAGAAAAACCTTTAACTGAGAAATATGGAAACCGTCTTAATTT
  TCCATTGGCTATGATGGAATTAATATTGTATTTTAAAAATGCATATTGATCACTATAATTCTAAAACAAT
  TTTTTAAATAAACCAGCAGGTTGCTAAAAGAAGGCATTTTATCTAAAGTTATTTTAATAGGTGGTATAGC
  AGTAATTTTAAATTTAAGAGTTGCTTTTACAGTTAACAATGGAATATGCCTTCTCTGCTATGTCTGAAAA
  TAGAAGCTATTTATTATGAGCTTCTACAGGTATTTTTAAATAGAGCAAGCATGTTGAATTTAAAATATGA
  ATAACCCCACCCAACAATTTTCAGTTTATTTTTTGCTTTGGTCGAACTTGGTGTGTGTTCATCACCCATC
  AGTTATTTGTGAGGGTGTTTATTCTATATGAATATTGTTTCATGTTTGTATGGGAAAATTGTAGCTAAAC
  ATTTCATTGTCCCCAGTCTGCAAAAGAAGCACAATTCTATTGCTTTGTCTTGCTTATAGTCATTAAATCA
  TTACTTTTACATATATTGCTGTTACTTCTGCTTTCTTTAAAAATATAGTAAAGGATGTTTTATGAAGTCA
  CAAGATACATATATTTTTATTTTGACCTAAATTTGTACAGTCCCATTGTAAGTGTTGTTTCTAATTATAG
  ATGTAAAATGAAATTTCATTTGTAATTGGAAAAAATCCAATAAAAAGGATATTCATTTAGAAAATAGCTA
  AGATCTTTAATAAAAATTTGATATGAAAAGCACAATGTGCAGAAGTTATGGAAAACCTATAGAGGATTAC
  AACAGGTAAACGTTAAAGAGAATACATTGCTGACTTATAGTGATGTGGCTAAGAAGTACATGCTTTGTTG
  TAAAATTGCTTGAAAGCCCATTGAAAGATGTATCTGTTTATTTACAGTCTTTGAAGTAAAAGTTACCAAT
  GTTTGCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005180.6
• Summary: 'This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]'
• Locus ID: 648