TIAM1 (NM_003253) Human 3' UTR Clone

CAT#: SC217254

3`UTR clone of T-cell lymphoma invasion and metastasis 1 (TIAM1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TIAM1
• Synonyms: FLJ36302
• ACCN: NM_003253
• Insert Size: 1958
• Sequence Data:
  Insert Sequence

  >SC217254 3'UTR clone of NM_003253
  The sequence shown below is from the reference sequence of NM_003253. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTCCAGGAAACTGAACACTGAGATCTGACTGCGTCACCTGCCCCGTAGAGAATGTGTGTAGATACTTCCT
  GCCCTAACTCTGCCCACCCTCCTGTACCGTCGACAAGAATGTCCCCTTAGGTCGCGCTCTTGCACACACG
  GTTTTGGCAGCTGACTTGGTTCTGAAGCCATGTAGCCACCCAACTTTGTCATTTTCAACAACATCAGAAA
  GAATTGATCAGAATCCCAAATAAGCTTGAGTCCTATCTTCTGTATATTACTAAGGGCTTTTATTTATTCT
  CAATAAATCAGGGCCTGAACAATTAAAAGAAAAAAGATTCTATAGCACTGGAAAGCAAATCACCCCAGGA
  GTTAACGGATGTACAACAGATTAATTTAAGGGATAGTAGCACACACACGATCCTTCTATCTGAAATCAGT
  CTCCTAGCTGGGGAAACCTCTTTCACACACAAAATGAAATGTGTACAGCTTGCCGTGTTCTGACTGTACC
  CTTCCCTCTTCCATGTCTGAGAATCTCCGTGTATTTTAAGAATGTGTGAGGAGAGGGTGGCGATTCATGT
  TTCAATGAGCCTCTTTTTTTTTTTCCTTCCTGTTTTGGTCTATGGCTGGTCTTACTCTGTGTCCATGTTC
  GGAAGCTCTAGTTTTGCATAGAATTATAGAGATGCCAAACTCTTTGAAAAGAGATCCAAATTTATCGCTT
  GAGAGAAAGAAAAGAAACACTATTTTTTGTATTTTACCTGAGATACAGGGGCACAAATAGATGAGAATTT
  TACAGTGTTAGTGTATGTATCCCTGAGCCTAAAAAATGAGGATATAACCTTTTACAGAGAGAGTGAGGCG
  TGGTGGTTTTATATTTATATATGAAAGGCCAGCAAGCTCATGCGAAGGATATACTTTTCTTCCAAAAAGC
  GGATTTTTTTTTTTTAATGTTTGAATCTATATTTGAGATGGGAGTTTGGTTGGATTAAACATGACACCCC
  GGTGGGCGGTGTGTGTGTCTGTTGCACATGGCAGGGAGGGGAGCCTCCTTCTCATGGGGTTGCCATGGTG
  ATCATTGGTTTTTCCATCAAAATTGCATCTTCATCCATAGATTACCTTCCCCTTCCCTGACAGTCCATAA
  CCAAACCTTTAAACAGAACAACCTCTTTAAAAACTTCTCTTGTGTTTAACACTTTCTTCATGCCAACGAA
  ACAGGGTAAACATGCTCAAAACATTAACAGTCTAAACAGATATCCAAATACTAAGAAGAAAAACAAGTTA
  TAGCACTTTCAATTTTTTTTTTTTTTTTAAAAAAAGGTTTATAGCTTTTTCTTTTCCCATGTCACAATGT
  CCACTTCCTAAGAAGGGTTTAAAATACTATGAAAACTTTCTTTTTGGGGAAAATATCTATTTGGTGTTTG
  ACACATCAGTAGGTACTTTAAAGACCTGAATTTTATAGTAGCTTTAGGAGTTATATTTTATAAAAATCAG
  TTATGACTTTATATTTCCAGACAATAGAGAGTTCAGTACATCATGCTCTTGTGCCTCTGCCTGCTTTTCC
  TGCGTTCCCACCCTGTATTCCCCCCGCCTTTCGGGTTTCCAGGGCTTCGAGCTTGATCTTTTGAAAGTTT
  TATTCTATTAAATTTTTGCTATATCTTCTGGTTTTCTGAAAAAGCTTTAGAATGGTTTCTATACCCTTTG
  TATCACTGCATTTTTCCATATCATCTCCGGTTCGATCGCGTCCAGATGGAAAACGGAAGCAGAGGCTTCT
  AATCGTCGCATTTACTGGCTCCAGTGCAACACATCCATCTGAAAACACTCGGAAGTCTGGTGCTTGGAGA
  GGGTGCCATTGTCTCTTGTACATAAGGTCATGACGTGTCTATGTCAAAAGTTCTTATATATTTCTTTTAT
  AAGCTGAAAGAAGGTCTATTTTTATGTTTTTAGGTCTATGAATGGAACGTTGTAAATGCTTGTCAAAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_003253.2
• Summary: This gene encodes a RAC1-specific guanine nucleotide exchange factor (GEF). GEFs mediate the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). The binding of GTP induces a conformational change in RAC1 that allows downstream effectors to bind and transduce a signal. This gene thus regulates RAC1 signaling pathways that affect cell shape, migration, adhesion, growth, survival, and polarity, as well as influencing actin cytoskeletal formation, endocytosis, and membrane trafficking. This gene thus plays an important role in cell invasion, metastasis, and carcinogenesis. In addition to RAC1, the encoded protein activates additional Rho-like GTPases such as CDC42, RAC2, RAC3 and RHOA. This gene encodes multiple protein isoforms that experience a diverse array of intramolecular, protein-protein, and phosphorylation interactions as well as phosphoinositide binding. Both the longer and shorter isoforms have C-terminal Dbl homology (DH) and pleckstrin homology (PH) domains while only the longer isoforms of this gene have the N-terminal myristoylation site and the downstream N-terminal PH domain, ras-binding domain (RBD), and PSD-95/DlgA/ZO-1 (PDZ) domain. [provided by RefSeq, Jul 2017]
• Locus ID: 7074