Rnaseh1 (BC019411) Mouse Untagged Clone

CAT#: MC206312

Rnaseh1 (untagged) - Mouse ribonuclease H1 (cDNA clone MGC:30220 IMAGE:5133942), (10ug)


Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Rnaseh1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC019411
CGCGCTGGCGTGAGTGATGCGCTGGCTGCTGCCGCTGTCCCGCACAGTGACACTGGCCGTCGTACGCCTG AGGCGAGGCATTTGCGGGCTCGGGATGTTCTATGCGGTGAGGAGAGGCCGCAGGACCGGAGTCTTCCTGA GTTGGAGTGAGTGCAAAGCCCAGGTGGACCGGTTTCCTGCTGCCAGGTTTAAGAAATTTGCCACAGAAGA TGAGGCCTGGGCCTTTGTCAGGAGCTCTTCAAGCCCGGATGGTTCAAAAGGGCAGGAAAGTGCACATGAG CAGAAGTCACAGGTGAAGACCAGCAAGCGGCCTCGGGAGCCCCTGGGTGAAGGGGAAGAACTTCCAGAGC CAGGGCCAAAGCACACACGACAGGACACCGAGCCCGCTGCTGTAGTGAGCAAGGACACGTTTTCTTATAT GGGAGAGTCAGTCATTGTCTACACGGATGGCTGTTGCTCCAGTAATGGACGGAAGCGGGCACGAGCAGGA ATTGGCGTTTACTGGGGCCCAGGCCACCCCTTAAATGTAGGTATAAGGCTTCCTGGGCGACAGACAAACC AGAGGGCCGAGATCCATGCAGCCTGCAAGGCCATCATGCAAGCCAAGGCTCAGAACATCAGCAAGCTGGT TCTGTACACAGACAGCATGTTCACCATCAATGGGATAACTAACTGGGTTCAGGGCTGGAAGAAGAATGGC TGGAGAACAAGTACAGGGAAAGATGTGATCAACAAGGAGGACTTCATGGAGCTGGACGAGCTCACTCAGG GCATGGACATCCAATGGATGCACATTCCTGGTCACTCAGGATTTGTGGGCAATGAAGAGGCCGACAGACT GGCACGGGAAGGAGCGAAGCAGTCTGAGGACTGAGCAGAGGAGCTGCTGCCGACTCAGACCCAAGGCAGA GGCTGTTTCTTTACCCTGCCGTGCACTGAGCTGTGCATGGAGGACACAGTGTAACCTGTGGCACAGCTTG GTGAGACGTGCTTACATAATGGAAAGGAAGTGTTAAATCCTGCTTTTGTGAGATTTCAGGGTCTGTCCCA AAATGCTCCAGCTGGATTTTAGTAGGAGTCAGTGAGACCCTGGTCAGTACGTGTGGCCTAAAAGTTCCAG AAGGCACAAGTGCAAGACTCCTGCCCCTGCAGCTGCCCTCTATCTCTGTGCATCTGAGATAATGTGGCCA CATGGGACACAGCCTACACTCAGGTGGCCTTGACTCCTGGATCACCTTGAAGGAACAGCTGAGACCTTTA AAAATCAATATATTACTAATAGATTTCTTTGCCTATTTTTTCTTAAAAATACTGTATAAATTTAAAACAT GTTGAAATTATCCTTATTTATTTTTTATAATAAAAGTATATAGGAATGACACAGAAAAAAAAAAAAAAAA AAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN BC019411
ORF Size 858 bp
Insert Size 858
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Reference Data
RefSeq BC019411, AAH19411
RefSeq Size 1409
RefSeq ORF 858
Locus ID 19819
Gene Summary This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which encodes 7aa in mouse. An alternately spliced transcript variant has been found which is a candidate for nonsense-mediated mRNA decay (NMD). [provided by RefSeq, Nov 2013]

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