Mars (NM_001003913) Mouse Untagged Clone

CAT#: MC222485

Mars (untagged) - Mouse methionine-tRNA synthetase (Mars), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mars
• Synonyms: Metrs; Mtrns
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222485 representing NM_001003913
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGTTCGTGAGCGAGGGTTCCCCGGGGAGCCTGCCCGTGCTGGCTGCGGCCGCGAGGGCCCGGG
  GTCGGGCGGAGCTGCTCATCAGCACCGTAGGCCCCGAAGAGTGTGTGGTACCATTCCTTACCCGGCCTAA
  GGTCCCTGTCTTGCAGCTGGATAGTGGCAACTACCTCTTCTCTGCTAGTGCAATCTGCCGATATTTTTTT
  CTGTTATGTGGCTGGGAACAAGATGATCTCACCAACCAGTGGCTGGAATGGGAGGCAACAGAACTGCAGC
  CAGTTCTGTCTGCTGCCCTACACTGTCTAGTGGTTCAAGGCAAGAAAGGGGAAGATATACTTGGCCCACT
  TCGGAGAGTCCTGACTCACATTGATCACAGCTTGAGTCGTCAAAACTGTCCTTTCCTGGCTGGGGACACA
  GAATCTCTAGCTGACATTGTTTTGTGGGGAGCACTGTATCCTTTACTGCAAGACCCAGCTTACCTCCCTG
  AGGAGTTGGGTGCCCTGCAAAGTTGGTTCCAGACACTGAGTACCCAGGAACCGTGTCAGCGAGCTGCAGA
  GACGGTGCTAAAACAGCAGGGTGTCCTGGCACTTCGTCTGTACCTCCAGAAACAGCCACAGCCTCAGCCC
  CCGCCTCCTGAGGGGAGAACTGTCAGCAACGAGCTGGAGGAAGAGGAACTGGCTACCTTGTCTGAGGAGG
  ACATCGTTACAGCTGTTGCCGCGTGGGAGAAGGGTCTGGAAAGCCTGCCTCCGCTAAAGCTCCAGCAGCA
  TCCAGTGTTGCCTGTGCCTGGAGAGAGGAATGTTCTCATCACCAGTGCCCTCCCCTATGTCAACAATGTC
  CCCCACCTTGGAAACATCATTGGCTGTGTGCTCAGTGCTGATGTCTTTGCAAGGTATTGTCGCCTTCGCC
  AGTGGAATACCCTCTATCTGTGTGGTACAGATGAGTATGGTACTGCGACAGAGACCAAGGCCATGGAGGA
  GGGCCTAACCCCACGGGAAATCTGTGACAAGTACCATGCCATCCATGCTGACATCTACCGCTGGTTCGGC
  ATATCGTTCGATACTTTCGGGCGCACTACCACTCCTCAGCAGACCAAAATCACCCAGGACATCTTCCAGA
  GGTTGCTGACCCGGGGGTTTGTGCTGCGAGATACTGTGGAGCAGCTTCGGTGTGAGCGGTGTGCACGTTT
  CCTGGCTGACCGCTTTGTGGAGGGTGTGTGTCCCTTCTGTGGCTATGAAGAGGCCCGAGGTGACCAGTGT
  GACAGGTGTGGCAAGCTCATCAATGCCATTGAGCTCAAGAAACCACAGTGCAAAATCTGCCGCTCCTGCC
  CTGTGGTGAGGTCCTCACAGCACCTGTTTCTAGACTTGCCTAAGTTGGAAAAGCGTCTGGAGGACTGGTT
  GGGGAAGACAGTGCCTGGCAGTGACTGGACACCCAATGCCAGGTTCATTATACGTTCCTGGCTTCGAGAT
  GGCCTCAAGCCACGATGCATCACCAGAGACCTCAAATGGGGAACGCCTGTGCCCTTGGAAGGTTTTGAGG
  ACAAGGTATTTTACGTCTGGTTTGATGCTACTATTGGCTACGTGTCCATCACAGCCAACTACACAGACCA
  ATGGGAGAAATGGTGGAAGAACCCAGAACAAGTGGACCTTTACCAGTTCATGGCCAAAGACAATGTTCCC
  TTCCATGGCTTGGTCTTTCCGTGTTCAGTCCTAGGAGCTGAGGACAACTACACCCTGGTCAAGCACATCA
  TTGCTACAGAGTACCTGAACTATGAGGATGGGAAATTCTCTAAGAGCCGGGGCATAGGAGTGTTTGGAGA
  CATGGCCAAGGATACAGGAATCCCTGCTGACATCTGGCGATTCTATCTGCTATACATTCGGCCTGAGGGC
  CAGGACAGTGCCTTCTCCTGGACAGACTTGTTGATTAAAAACAATTCTGAGCTGCTCAACAACCTGGGCA
  ACTTCATCAACAGAGCTGGCATGTTTGTTTCTAAGTTTTTTGGCGGTTGTGTGCCTGAGATGGCGCTAAC
  CCCTGATGACAGACGCCTGGTGGCCCATGTCTCTTGGGAACTCCAGCACTATCACCAGCTGTTGGAAAAG
  GTTCGGATCCGGGATGCCTTGCGCAGTATCCTCACCATATCTCGCCATGGCAACCAATACATTCAAGTGA
  ATGAGCCCTGGAAACGGATTAAAGGTGGTGAGATGGACAGGCAGCGGGCAGGCACAGTGACAGGCATGGC
  AGTGAACATGGCTGCCTTGCTGTCTGTCATGCTGCAGCCATACATGCCCACAGTCAGCTCTACCATCCAG
  ACCCAGCTGCAGCTCCCACCTGCAGCCTGCCGCATCCTTGCCACAAGCTTCATTTGTACCTTGCCAGCAG
  GCCACCGAATTGGCACAGTCAGTCCTTTGTTCCAAAAACTGGAAAATGACCAGATTGAAAATTTGAGGCA
  GCGCTTTGGAGGGGGTCAGGCTAAAGGCTCCCCCAAGCCAGCAGCTGTGGAGGCAGTTACAGCAGCAGGC
  TCGCAGCACATACAAACGCTGACGGATGAGGTGACCAAGCAGGGCAACGTCGTCCGGGAACTGAAAGCAC
  AGAAGGCAGACAAGAACCAGGTTGCTGCAGAGGTGGCTAAACTCTTGGATCTAAAGAAACAGTTGGCTTT
  GGCTGAGGGGAAACCCATTGAAACTCCTAAAGGCAAGAAGAAAAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003913
• Insert Size: 2709 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003913.2, NP_001003913.1
• RefSeq Size: 2948 bp
• RefSeq ORF: 2709 bp
• Locus ID: 216443
• Cytogenetics: 10 D3
• Gene Summary: The encoded protein belongs to the class I family of tRNA synthetases, a class of enzymes that charge tRNAs with their cognate amino acids. The related human gene product is essential for the translation initiation of mRNAs. This gene has an overlapping 3' UTR tail-to-tail arrangement with an adjacent gene on the opposite strand that encodes an inhibitor of the CCAAT/enhancer-binding protein's DNA binding activity. This arrangement, conserved in human and mouse, may be involved in mRNA stability and possible functional and regulatory interaction of these adjacent overlapping genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.