Mars (NM_001003913) Mouse Tagged ORF Clone

CAT#: MR211112

Mars (Myc-DDK-tagged) - Mouse methionine-tRNA synthetase (Mars), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mars
• Synonyms: Metrs; Mtrns
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211112 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGTTCGTGAGCGAGGGTTCCCCGGGGAGCCTGCCCGTGCTGGCTGCGGCCGCGAGGGCCCGGG
  GTCGGGCGGAGCTGCTCATCAGCACCGTAGGCCCCGAAGAGTGTGTGGTACCATTCCTTACCCGGCCTAA
  GGTCCCTGTCTTGCAGCTGGATAGTGGCAACTACCTCTTCTCTGCTAGTGCAATCTGCCGATATTTTTTT
  CTGTTATGTGGCTGGGAACAAGATGATCTCACCAACCAGTGGCTGGAATGGGAGGCAACAGAACTGCAGC
  CAGTTCTGTCTGCTGCCCTACACTGTCTAGTGGTTCAAGGCAAGAAAGGGGAAGATATACTTGGCCCACT
  TCGGAGAGTCCTGACTCACATTGATCACAGCTTGAGTCGTCAAAACTGTCCTTTCCTGGCTGGGGACACA
  GAATCTCTAGCTGACATTGTTTTGTGGGGAGCACTGTATCCTTTACTGCAAGACCCAGCTTACCTCCCTG
  AGGAGTTGGGTGCCCTGCAAAGTTGGTTCCAGACACTGAGTACCCAGGAACCGTGTCAGCGAGCTGCAGA
  GACGGTGCTAAAACAGCAGGGTGTCCTGGCACTTCGTCTGTACCTCCAGAAACAGCCACAGCCTCAGCCC
  CCGCCTCCTGAGGGGAGAACTGTCAGCAACGAGCTGGAGGAAGAGGAACTGGCTACCTTGTCTGAGGAGG
  ACATCGTTACAGCTGTTGCCGCGTGGGAGAAGGGTCTGGAAAGCCTGCCTCCGCTAAAGCTCCAGCAGCA
  TCCAGTGTTGCCTGTGCCTGGAGAGAGGAATGTTCTCATCACCAGTGCCCTCCCCTATGTCAACAATGTC
  CCCCACCTTGGAAACATCATTGGCTGTGTGCTCAGTGCTGATGTCTTTGCAAGGTATTGTCGCCTTCGCC
  AGTGGAATACCCTCTATCTGTGTGGTACAGATGAGTATGGTACTGCGACAGAGACCAAGGCCATGGAGGA
  GGGCCTAACCCCACGGGAAATCTGTGACAAGTACCATGCCATCCATGCTGACATCTACCGCTGGTTCGGC
  ATATCGTTCGATACTTTCGGGCGCACTACCACTCCTCAGCAGACCAAAATCACCCAGGACATCTTCCAGA
  GGTTGCTGACCCGGGGGTTTGTGCTGCGAGATACTGTGGAGCAGCTTCGGTGTGAGCGGTGTGCACGTTT
  CCTGGCTGACCGCTTTGTGGAGGGTGTGTGTCCCTTCTGTGGCTATGAAGAGGCCCGAGGTGACCAGTGT
  GACAGGTGTGGCAAGCTCATCAATGCCATTGAGCTCAAGAAACCACAGTGCAAAATCTGCCGCTCCTGCC
  CTGTGGTGAGGTCCTCACAGCACCTGTTTCTAGACTTGCCTAAGTTGGAAAAGCGTCTGGAGGACTGGTT
  GGGGAAGACAGTGCCTGGCAGTGACTGGACACCCAATGCCAGGTTCATTATACGTTCCTGGCTTCGAGAT
  GGCCTCAAGCCACGATGCATCACCAGAGACCTCAAATGGGGAACGCCTGTGCCCTTGGAAGGTTTTGAGG
  ACAAGGTATTTTACGTCTGGTTTGATGCTACTATTGGCTACGTGTCCATCACAGCCAACTACACAGACCA
  ATGGGAGAAATGGTGGAAGAACCCAGAACAAGTGGACCTTTACCAGTTCATGGCCAAAGACAATGTTCCC
  TTCCATGGCTTGGTCTTTCCGTGTTCAGTCCTAGGAGCTGAGGACAACTACACCCTGGTCAAGCACATCA
  TTGCTACAGAGTACCTGAACTATGAGGATGGGAAATTCTCTAAGAGCCGGGGCATAGGAGTGTTTGGAGA
  CATGGCCAAGGATACAGGAATCCCTGCTGACATCTGGCGATTCTATCTGCTATACATTCGGCCTGAGGGC
  CAGGACAGTGCCTTCTCCTGGACAGACTTGTTGATTAAAAACAATTCTGAGCTGCTCAACAACCTGGGCA
  ACTTCATCAACAGAGCTGGCATGTTTGTTTCTAAGTTTTTTGGCGGTTGTGTGCCTGAGATGGCGCTAAC
  CCCTGATGACAGACGCCTGGTGGCCCATGTCTCTTGGGAACTCCAGCACTATCACCAGCTGTTGGAAAAG
  GTTCGGATCCGGGATGCCTTGCGCAGTATCCTCACCATATCTCGCCATGGCAACCAATACATTCAAGTGA
  ATGAGCCCTGGAAACGGATTAAAGGTGGTGAGATGGACAGGCAGCGGGCAGGCACAGTGACAGGCATGGC
  AGTGAACATGGCTGCCTTGCTGTCTGTCATGCTGCAGCCATACATGCCCACAGTCAGCTCTACCATCCAG
  ACCCAGCTGCAGCTCCCACCTGCAGCCTGCCGCATCCTTGCCACAAGCTTCATTTGTACCTTGCCAGCAG
  GCCACCGAATTGGCACAGTCAGTCCTTTGTTCCAAAAACTGGAAAATGACCAGATTGAAAATTTGAGGCA
  GCGCTTTGGAGGGGGTCAGGCTAAAGGCTCCCCCAAGCCAGCAGCTGTGGAGGCAGTTACAGCAGCAGGC
  TCGCAGCACATACAAACGCTGACGGATGAGGTGACCAAGCAGGGCAACGTCGTCCGGGAACTGAAAGCAC
  AGAAGGCAGACAAGAACCAGGTTGCTGCAGAGGTGGCTAAACTCTTGGATCTAAAGAAACAGTTGGCTTT
  GGCTGAGGGGAAACCCATTGAAACTCCTAAAGGCAAGAAGAAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211112 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRLFVSEGSPGSLPVLAAAARARGRAELLISTVGPEECVVPFLTRPKVPVLQLDSGNYLFSASAICRYFF
  LLCGWEQDDLTNQWLEWEATELQPVLSAALHCLVVQGKKGEDILGPLRRVLTHIDHSLSRQNCPFLAGDT
  ESLADIVLWGALYPLLQDPAYLPEELGALQSWFQTLSTQEPCQRAAETVLKQQGVLALRLYLQKQPQPQP
  PPPEGRTVSNELEEEELATLSEEDIVTAVAAWEKGLESLPPLKLQQHPVLPVPGERNVLITSALPYVNNV
  PHLGNIIGCVLSADVFARYCRLRQWNTLYLCGTDEYGTATETKAMEEGLTPREICDKYHAIHADIYRWFG
  ISFDTFGRTTTPQQTKITQDIFQRLLTRGFVLRDTVEQLRCERCARFLADRFVEGVCPFCGYEEARGDQC
  DRCGKLINAIELKKPQCKICRSCPVVRSSQHLFLDLPKLEKRLEDWLGKTVPGSDWTPNARFIIRSWLRD
  GLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYVSITANYTDQWEKWWKNPEQVDLYQFMAKDNVP
  FHGLVFPCSVLGAEDNYTLVKHIIATEYLNYEDGKFSKSRGIGVFGDMAKDTGIPADIWRFYLLYIRPEG
  QDSAFSWTDLLIKNNSELLNNLGNFINRAGMFVSKFFGGCVPEMALTPDDRRLVAHVSWELQHYHQLLEK
  VRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEMDRQRAGTVTGMAVNMAALLSVMLQPYMPTVSSTIQ
  TQLQLPPAACRILATSFICTLPAGHRIGTVSPLFQKLENDQIENLRQRFGGGQAKGSPKPAAVEAVTAAG
  SQHIQTLTDEVTKQGNVVRELKAQKADKNQVAAEVAKLLDLKKQLALAEGKPIETPKGKKKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003913
• ORF Size: 2709 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003913.1, NM_001003913.2, NP_001003913.1
• RefSeq Size: 2948 bp
• RefSeq ORF: 2709 bp
• Locus ID: 216443
• Cytogenetics: 10 D3
• MW: 101.4 kDa
• Gene Summary: The encoded protein belongs to the class I family of tRNA synthetases, a class of enzymes that charge tRNAs with their cognate amino acids. The related human gene product is essential for the translation initiation of mRNAs. This gene has an overlapping 3' UTR tail-to-tail arrangement with an adjacent gene on the opposite strand that encodes an inhibitor of the CCAAT/enhancer-binding protein's DNA binding activity. This arrangement, conserved in human and mouse, may be involved in mRNA stability and possible functional and regulatory interaction of these adjacent overlapping genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]