Runx2 (NM_001145920) Mouse Tagged ORF Clone

CAT#: MG227541

Runx2 (GFP-tagged) - Mouse runt related transcription factor 2 (Runx2) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Runx2
• Synonyms: AML3; Cbf; Cbfa-1; Cbfa1; LS3; Osf2; Pebp2a1; PEBP2aA; Pebpa2a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227541 representing NM_001145920
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTATTCCTGTAGATCCGAGCACCAGCCGGCGCTTCAGCCCCCCCTCCAGCAGCCTGCAGCCCGGCA
  AGATGAGCGACGTGAGCCCGGTGGTGGCTGCGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAGCA
  GCAGCAACAACAGCAACAGCAACAACAGCAGCAGCAGCAGCAGCAGCAGGAGGCGGCCGCAGCAGCAGCG
  GCGGCAGCGGCGGCGGCAGCAGCGGCGGCGGCCGCAGTGCCCCGATTGAGGCCGCCGCACGACAACCGCA
  CCATGGTGGAGATCATCGCGGACCACCCGGCCGAACTGGTCCGCACCGACAGTCCCAACTTCCTGTGCTC
  CGTGCTGCCCTCGCACTGGCGGTGCAACAAGACCCTGCCCGTGGCCTTCAAGGTTGTAGCCCTCGGAGAG
  GTACCAGATGGGACTGTGGTTACCGTCATGGCCGGGAATGATGAGAACTACTCCGCCGAGCTCCGAAATG
  CCTCCGCTGTTATGAAAAACCAAGTAGCCAGGTTCAACGATCTGAGATTTGTGGGCCGGAGCGGACGAGG
  CAAGAGTTTCACCTTGACCATAACAGTCTTCACAAATCCTCCCCAAGTGGCCACTTACCACAGAGCTATT
  AAAGTGACAGTGGACGGTCCCCGGGAACCAAGAAGGCACAGACAGAAGCTTGATGACTCTAAACCTAGTT
  TGTTCTCTGATCGCCTCAGTGATTTAGGGCGCATTCCTCATCCCAGTATGAGAGTAGGTGTCCCGCCTCA
  GAACCCACGGCCCTCCCTGAACTCTGCACCAAGTCCTTTTAATCCACAAGGACAGAGTCAGATTACAGAT
  CCCAGGCAGGCACAGTCTTCCCCACCGTGGTCCTATGACCAGTCTTACCCCTCCTATCTGAGCCAGATGA
  CATCCCCATCCATCCACTCCACCACGCCGCTGTCTTCCACACGGGGCACCGGGCTACCTGCCATCACTGA
  CGTGCCCAGGCGTATTTCAGATGATGACACTGCCACCTCTGACTTCTGCCTCTGGCCTTCCTCTCTCAGT
  AAGAAGAGCCAGGCAGGTGCTTCAGAACTGGGCCCTTTTTCAGACCCCAGGCAGTTCCCAAGCATTTCAT
  CCCTCACTGAGAGCCGCTTCTCCAACCCACGAATGCACTACCCAGCCACCTTTACCTACACCCCGCCAGT
  CACGTCAGGCATGTCCCTCGGCATGTCCGCCACCACTCACTACCACACGTACCTGCCACCACCCTACCCC
  GGCTCTTCCCAAAGCCAGAGTGGACCCTTCCAGACCAGCAGCACTCCATATCTCTACTATGGTACTTCGT
  CAGCATCCTATCAGTTCCCAATGGTACCCGGGGGAGACCGGTCTCCTTCCAGGATGGTCCCACCATGCAC
  CACCACCTCGAATGGCAGCACGCTATTAAATCCAAATTTGCCTAACCAGAATGATGGTGTTGACGCTGAC
  GGAAGCCACAGCAGTTCCCCAACTGTTTTGAATTCTAGCGGCAGAATGGATGAGTCTGTTTGGCGGCCAT
  AT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227541 representing NM_001145920
  Red=Cloning site Green=Tags(s)
  
  MRIPVDPSTSRRFSPPSSSLQPGKMSDVSPVVAAQQQQQQQQQQQQQQQQQQQQQQQQQQQQQEAAAAAA
  AAAAAAAAAAAAVPRLRPPHDNRTMVEIIADHPAELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGE
  VPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAI
  KVTVDGPREPRRHRQKLDDSKPSLFSDRLSDLGRIPHPSMRVGVPPQNPRPSLNSAPSPFNPQGQSQITD
  PRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLPAITDVPRRISDDDTATSDFCLWPSSLS
  KKSQAGASELGPFSDPRQFPSISSLTESRFSNPRMHYPATFTYTPPVTSGMSLGMSATTHYHTYLPPPYP
  GSSQSQSGPFQTSSTPYLYYGTSSASYQFPMVPGGDRSPSRMVPPCTTTSNGSTLLNPNLPNQNDGVDAD
  GSHSSSPTVLNSSGRMDESVWRPY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145920
• ORF Size: 1542 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145920.1, NP_001139392.1
• RefSeq Size: 6463
• RefSeq ORF: 1545
• Locus ID: 12393
• Gene Summary: This gene encodes a member of the runt domain-containing family of transcription factors. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Sep 2015]