Efemp1 (BC031184) Mouse Tagged ORF Clone

CAT#: MR206053

Efemp1 (Myc-DDK-tagged) - Mouse epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (cDNA clone MGC:37612

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Efemp1
• Synonyms: MGC37612
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206053 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCAGTGGTGTGGTGCCTGGGGGTGGCTTCATGGCCAGTGCTACTGCAGTTGCTGGCCCTGAAG
  TTCAAACTGGCCGAAATAACTTTGTCATCCGAAGAAACCCAGCTGACCCTCAGCGCATCCCTTCCAACCC
  TTCCCACCGGATCCAGTGTGCAGCAGGCTATGAACAGAGTGAGCATAATGTGTGCCAAGATATTGATGAG
  TGCACCTCAGGGACTCACAATTGTAGAACGGACCAAGTATGCATCAATTTACGAGGTTCCTTCACATGTC
  AGTGTCTTCCTGGGTATCAGAAGCGAGGTGAACAGTGTGTGGATATAGATGAATGCACAGTGCCTCCATA
  TTGCCACCAAAGATGTGTTAACACACCTGGTTCCTTCTACTGCCAGTGCAGTCCAGGGTTTCAGCTGGCA
  GCAAACAACTACACTTGTGTGGATATAAATGAATGTGATGCCAGCAACCAGTGTGCTCAACAATGCTACA
  ACATTCTTGGCTCATTCATCTGTCAGTGTAATCAAGGATATGAACTAAGCAGTGACAGACTCAACTGTGA
  AGACATCGACGAATGCAGAACCTCAAGCTACCTATGCCAATATCAATGTGTCAATGAACCTGGGAAGTTC
  TCATGTATGTGCCCACAGGGTTACGAAGTGGTGCGCAGCAGAACCTGTCAGGATATAAATGAATGTGAGA
  CCACCAATGAATGCCGAGAAGATGAGATGTGCTGGAATTACCATGGGGGCTTCCGCTGTTACCCACGAAA
  CCCATGTCAAGATCACTATGTTCTAACATCAGAAAACCGATGTGTTTGCCCAGTCTCAAACACTATGTGC
  CGGGAGCTGCCCCAGTCCATTGTCTACAAATACATGAGCATCCGATCTGACAGGTCCGTGCCTTCAGACA
  TCTTCCAGATACAGGCAACAATGATTTATGCAAACACCATTAATACTTTTCGGATTAAATCTGGAAATGA
  AAATGGAGAGTTCTACCTACGACAAACAAGCCCTGTGAGTGCAATGCTGGTGCTTGTGAAGTCTCTATCA
  GGACCAAGAGAATACATCGTGGACCTGGAGATGCTGACAGTCAGTAGTATAGGAACCTTCCGCACAAGCT
  CTGTGTTAAGATTGACAATAATAGTGGGGCCATTTTCATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206053 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATSGVVPGGGFMASATAVAGPEVQTGRNNFVIRRNPADPQRIPSNPSHRIQCAAGYEQSEHNVCQDIDE
  CTSGTHNCRTDQVCINLRGSFTCQCLPGYQKRGEQCVDIDECTVPPYCHQRCVNTPGSFYCQCSPGFQLA
  ANNYTCVDINECDASNQCAQQCYNILGSFICQCNQGYELSSDRLNCEDIDECRTSSYLCQYQCVNEPGKF
  SCMCPQGYEVVRSRTCQDINECETTNECREDEMCWNYHGGFRCYPRNPCQDHYVLTSENRCVCPVSNTMC
  RELPQSIVYKYMSIRSDRSVPSDIFQIQATMIYANTINTFRIKSGNENGEFYLRQTSPVSAMLVLVKSLS
  GPREYIVDLEMLTVSSIGTFRTSSVLRLTIIVGPFSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC031184
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031184, AAH31184
• RefSeq Size: 1677 bp
• RefSeq ORF: 1163 bp
• Locus ID: 216616
• MW: 43.3 kDa