Efemp1 (BC031184) Mouse Tagged Lenti ORF Clone

CAT#: MR206053L3

  • LentiORF®

Lenti ORF clone of Efemp1 (Myc-DDK-tagged) - Mouse epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (cDNA clone MGC:37612


  "BC031184" in other vectors (3)


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Reconstitution Protocol

USD 528.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (2)
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Lenti-vpak packaging kit - packaging plasmids and transfection reagent
    • 10 reactions

USD 649.00

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Efemp1
Synonyms MGC37612
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(MR206053).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN BC031184
ORF Size 1161 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC031184, AAH31184
RefSeq Size 1677 bp
RefSeq ORF 1163 bp
Locus ID 216616
Cytogenetics 11 A3.3
Gene Summary Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth (By similarity).[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.