RIOK2 (NM_018343) Human Tagged ORF Clone

CAT#: RC201484

RIOK2 (Myc-DDK-tagged)-Human RIO kinase 2 (RIOK2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402668) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201484 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIOK2
• Synonyms: RIO2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201484 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAAGTGAATGTGGCCAAGTTGCGTTACATGAGCCGAGATGACTTCAGGGTCTTGACCGCGGTTG
  AAATGGGCATGAAGAACCATGAAATTGTTCCCGGCAGTTTGATTGCTTCTATAGCCAGCCTTAAACATGG
  TGGCTGTAATAAAGTTTTAAGAGAATTAGTGAAACATAAACTCATAGCTTGGGAGCGTACCAAAACTGTC
  CAGGGCTATCGGTTGACAAATGCAGGATATGATTACCTAGCTTTGAAAACACTTTCTTCTAGGCAAGTAG
  TTGAGTCTGTTGGAAACCAGATGGGTGTTGGCAAAGAATCAGATATTTACATTGTTGCAAATGAAGAAGG
  ACAACAATTTGCATTAAAGCTTCACAGACTAGGAAGAACCTCGTTTCGAAATTTGAAAAACAAACGCGAT
  TATCATAAATATAGGCACAATGTGTCATGGCTATATTTATCTCGTCTCTCTGCCATGAAGGAATTTGCCT
  ATATGAAGGCATTGTATGAGAGGAAATTTCCAGTTCCAAAGCCAATTGATTACAATCGTCATGCAGTGGT
  CATGGAACTCATAAATGGTTATCCACTATGTCAGATACACCATGTTGAAGATCCTGCATCAGTATATGAT
  GAAGCTATGGAACTAATTGTCAAACTTGCAAATCATGGGCTGATTCATGGAGATTTTAATGAATTTAATC
  TCATTTTGGATGAAAGTGACCATATCACCATGATTGATTTTCCACAGATGGTTTCAACTTCTCATCCCAA
  TGCTGAGTGGTATTTTGACAGAGATGTTAAATGCATTAAAGATTTCTTTATGAAACGTTTCAGCTACGAA
  AGTGAGCTTTTTCCAACTTTTAAGGATATCAGGAGAGAAGACACTCTTGATGTGGAGGTTTCTGCCAGTG
  GCTACACAAAGGAAATGCAGGCAGATGATGAACTGCTTCATCCATTAGGTCCAGATGATAAAAATATTGA
  AACAAAAGAGGGATCTGAATTCTCATTTTCAGATGGAGAAGTGGCAGAAAAAGCAGAGGTTTACGGGTCA
  GAAAATGAAAGTGAACGGAACTGTCTAGAAGAATCAGAGGGCTGCTATTGCAGATCATCTGGAGACCCTG
  AACAAATAAAGGAAGACAGTTTATCAGAAGAGAGTGCTGATGCACGGAGTTTTGAAATGACTGAATTCAA
  TCAAGCTTTAGAAGAAATAAAAGGGCAGGTTGTTGAAAACAACTCTGTAACTGAATTTTCTGAGGAGAAA
  AACAGAACTGAAAATTACAACAGGCAAGATGGTCAGAGAGTTCAAGGAGGAGTCCCTGCTGGCTCTGACG
  AGTATGAAGATGAATGCCCTCATCTAATTGCCTTGTCGTCATTAAATAGAGAATTCAGGCCTTTCAGAGA
  TGAAGAAAATGTGGGAGCTATGAATCAGTATAGAACAAGAACTCTGAGTATCACTTCTTCAGGCAGTGCT
  GTAAGCTGTTCAACAATTCCTCCAGAACTGGTGAAACAGAAGGTGAAACGTCAGTTGACAAAACAGCAAA
  AATCAGCTGTCAGACGTCGATTGCAGAAAGGAGAAGCAAATATATTTACCAAGCAACGTAGGGAAAACAT
  GCAAAATATCAAATCAAGTTTGGAAGCAGCCAGCTTTTGGGGAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201484 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTV
  QGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRD
  YHKYRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYD
  EAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYE
  SELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGS
  ENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEK
  NRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSA
  VSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018343
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018343.1, NM_018343.2, NP_060813.1
• RefSeq Size: 3980 bp
• RefSeq ORF: 1659 bp
• Locus ID: 55781
• Domains: RIO
• Protein Families: Druggable Genome, Protein Kinase
• MW: 63.3 kDa
• Gene Summary: Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its catalytic activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA. [UniProtKB/Swiss-Prot Function]