RIOK2 (NM_018343) Human Untagged Clone

CAT#: SC323501

RIOK2 (untagged)-Kinase deficient mutant (D228A) of Human RIO kinase 2 (yeast) (RIOK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RIOK2
• Synonyms: RIO2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323501 sequence for NM_018343 edited (data generated by NextGen Sequencing)
  ATGGGGAAAGTGAATGTGGCCAAGTTGCGTTACATGAGCCGAGATGACTTCAGGGTCTTG
  ACCGCGGTTGAAATGGGCATGAAGAACCATGAAATTGTTCCCGGCAGTTTGATTGCTTCT
  ATAGCCAGCCTTAAACATGGTGGCTGTAATAAAGTTTTAAGAGAATTAGTGAAACATAAA
  CTCATAGCTTGGGAGCGTACCAAAACTGTCCAGGGCTATCGGTTGACAAATGCAGGATAT
  GATTACCTAGCTTTGAAAACACTTTCTTCTAGGCAAGTAGTTGAGTCTGTTGGAAACCAG
  ATGGGTGTTGGCAAAGAATCAGATATTTACATTGTTGCAAATGAAGAAGGACAACAATTT
  GCATTAAAGCTTCACAGACTAGGAAGAACCTCGTTTCGAAATTTGAAAAACAAACGCGAT
  TATCATAAACATAGGCACAATGTGTCTTGGCTTTATTTATCTCGTCTCTCTGCCATGAAG
  GAATTTGCCTATATGAAGGCATTGTATGAGAGGAAATTTCCAGTTCCAAAGCCAATTGAT
  TACAATCGTCATGCAGTGGTCATGGAACTCATAAATGGTTATCCACTATGTCAGATACAC
  CATGTTGAAGATCCTGCATCAGTATATGATGAAGCTATGGAACTAATTGTCAAACTTGCA
  AATCATGGGCTGATTCATGGAGCKTTTAATGAATTTAATCTCATTTTGGATGAAAGTGAC
  CATATCACCATGATTGATTTTCCACAGATGGTTTCAACTTCTCATCCCAATGCTGAGTGG
  TATTTTGACAGAGATGTTAAATGCATTAAAGATTTCTTTATGAAACGTTTCAGCTACGAA
  AGTGAGCTTTTTCCAACTTTTAAGGATATCAGGAGAGAAGACACTCTTGATGTGGAGGTT
  TCTGCCAGTGGCTACACAAAGGAAATGCAGGCAGATGATGAACTGCTTCATCCATTAGGT
  CCAGATGATAAAAATATTGAAACAAAAGAGGGATCTGAATTCTCATTTTCAGATGGAGAA
  GTGGCAGAAAAAGCAGAGGTTTACAGGTCAGAAAATGAAAGTGAACGGAACTGTCTAGAA
  GAATCAGAGGGCTGCTATTGCAGATCATCTGGAGACCCTGAACAAATAAAGGAAGACAGT
  TTATCAGAAGAGAGTGCTGATGCACGGAGTTTTGAAATGACTGAATTCAATCAAGCTTTA
  GAAGAAATAAAAGGGCAGGTTGTTGAAAACAACTCTGTAACTGAATTTTCTGAGGAGAAA
  AACAGAACTGAAAATTACAACAGGCAAGATGGTCAGAGAGTTCAAGGAGGAGTCCCTGCT
  GGCTCTGACGAGTATGAAGATGAATGCCCTCATCTAATTGCCTTGTCGTCATTAAATAGA
  GAATTCAGGCCTTTCAGAGATGAAGAAAATGTGGGAGCTATGAATCAGTATAGAACAAGA
  ACTCTGAGTATCACTTCTTCAGGCAGTGCTGTAAGCTGTTCAACAATTCCTCCAGAACTG
  GTGAAACAGAAGGTGAAACGTCAGTTGACAAAACAGCAAAAATCAGCTGTCAGACGTCGA
  TTGCAGAAAGGAGAAGCAAATATATTTACCAAGCAACGTAGGGAAAACATGCAAAATATC
  AAATCAAGTTTGGAAGCAGCTAGCTTTTGGGGAGAATAA
  
  Clone variation with respect to NM_018343.2
  447 a=>t;453 a=>t;683 a=>c;684 t=>k;1045 g=>a;1641 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_018343 unedited
  CCCGCCCGTTCAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGTCGGAGAGG
  CATCTGGGTTCGGACTGGGGCCGCCATGGGGAAAGTGAATGTGGCCAAGTTGCGTTACATGAGCCGAGAT
  GACTTCAGGGTCTTGACCGCGGTTGAAATGGGCATGAAGAACCATGAAATTGTTCCCGGCAGTTTGATTG
  CTTCTATAGCCAGCCTTAAACATGGTGGCTGTAATAAAGTTTTAAGAGAATTAGTGAAAACATAAACTCA
  TAGCTTGGGGAGCGTACCCAAAACTGTCCAGGGCTATCGGTTGACAAATGCAGGATATGATTACCTAGCT
  TTGAAAACCCTTTTCTTCTAGGCAAGTAGTTGAGTCCGGTGGAAACCCAGAGGGTGGTTGGCAAAGAATC
  AGATATTAAATGGTGCAAAGGAGAAGGGACAACATTTCCTTAAACCTCACACATAGAAAAACCCTGTTCC
  AATTTGAAAACACAACGCATATATAAACCTAGGCCATGTGGCTGGCTAATTATCTCTCATGGCCGAGAGA
  TTCCTATAAGCCTGTGAGAGAGAATCCGTTCAACGAAGTGATCACACACCACC
  
  Kinase domain sequence

  >SC323501 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GWCCCATGTTGAGWCCTGCATCAGTATATGATGAAGCTATGGAACTAATTGTCAAACTTGCAAATCATGG
  GCTGATTCATGGAGCGTTTAATGAATTTAATCTCATTTTGGATGAAAGTGACCATATCACCATGATTGAT
  TTTCCACAGATGGTTTCAACTTCTCATCCCAATGCTGAGTGGTATTTTGACAGAGATGTTAAATGCATTA
  AAGATTTCTTTATGAAACGTTTCAGCTACGAAAGTGAGCTTTTTC
  
• Restriction Sites: Please inquire
• ACCN: NM_018343
• ORF Size: 1659 bp
• Insert Size: 2110
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_018343.1, NP_060813.1
• RefSeq Size: 1832
• RefSeq ORF: 1659
• Locus ID: 55781
• Domains: RIO
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its catalytic activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.