CDC5L (NM_001253) Human Tagged ORF Clone
CAT#: RC201702
CDC5L (Myc-DDK-tagged)-Human CDC5 cell division cycle 5-like (S. pombe) (CDC5L)
"NM_001253" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CDC5L |
Synonyms | CDC5; CDC5-LIKE; CEF1; dJ319D22.1; PCDC5RP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201702 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCGAATTATGATCAAGGGGGGCGTATGGAGGAATACCGAGGATGAAATTCTGAAAGCAGCGGTAA TGAAATATGGGAAAAATCAGTGGTCTAGGATTGCCTCATTGCTGCATAGAAAATCAGCAAAGCAGTGCAA AGCCAGATGGTATGAATGGCTGGATCCAAGCATTAAGAAGACAGAATGGTCCAGAGAAGAAGAGGAAAAA CTCTTGCACTTGGCCAAGTTGATGCCAACTCAGTGGAGGACCATTGCTCCAATCATTGGAAGAACAGCGG CCCAGTGCTTAGAACACTATGAATTTCTTCTGGATAAAGCTGCCCAAAGAGACAATGAAGAGGAAACAAC AGATGATCCACGAAAACTTAAACCTGGAGAAATAGATCCAAATCCAGAAACAAAACCAGCGCGGCCTGAT CCAATTGATATGGATGAGGATGAACTTGAGATGCTTTCTGAAGCCAGAGCCCGCTTGGCTAATACTCAGG GAAAGAAGGCCAAGAGGAAAGCAAGAGAGAAACAATTGGAAGAAGCAAGACGTCTTGCTGCCCTCCAAAA AAGAAGAGAACTTCGAGCAGCTGGCATAGAAATTCAGAAGAAAAGAAAAAGGAAGAGAGGAGTTGATTAT AATGCCGAAATCCCATTTGAAAAAAAGCCTGCCCTTGGTTTTTATGATACTTCTGAGGAAAACTACCAAG CTCTTGACGCAGATTTCAGGAAATTAAGACAACAGGATCTTGATGGGGAGCTAAGATCTGAAAAAGAAGG AAGAGATAGAAAAAAAGACAAACAGCATTTGAAAAGGAAAAAAGAATCTGATTTACCATCAGCTATTCTT CAAACTAGTGGTGTTTCTGAATTTACTAAAAAGAGAAGCAAACTAGTACTTCCTGCCCCTCAGATTTCAG ATGCAGAACTCCAGGAAGTTGTAAAAGTAGGCCAAGCGAGTGAAATTGCACGTCAAACTGCCGAGGAATC TGGCATAACAAATTCTGCTTCCAGTACACTTTTGTCTGAGTACAATGTCACCAACAACAGCGTTGCTCTT AGAACACCACGAACACCAGCTTCCCAGGACAGAATTCTGCAGGAAGCCCAGAACCTCATGGCCCTCACCA ATGTGGACACCCCATTGAAAGGTGGACTTAATACCCCATTGCATGAGAGTGACTTCTCAGGTGTAACTCC ACAGCGACAAGTTGTACAGACTCCAAACACAGTTCTCTCTACTCCATTCAGGACTCCTTCTAATGGAGCT GAAGGGCTGACTCCCCGGAGTGGAACAACTCCCAAACCAGTTATTAACTCTACTCCGGGTAGAACTCCTC TTCGAGACAAGTTAAACATTAATCCCGAGGATGGAATGGCAGACTATAGTGATCCCTCTTACGTGAAGCA GATGGAAAGAGAATCCCGAGAACATCTCCGTTTAGGGTTGTTGGGCCTTCCTGCCCCTAAGAATGATTTT GAAATTGTTCTACCAGAAAATGCCGAGAAGGAGCTGGAAGAACGTGAAATAGATGATACTTACATTGAAG ATGCTGCTGATGTGGATGCTCGAAAGCAGGCCATACGAGATGCAGAGCGTGTAAAGGAAATGAAACGAAT GCATAAAGCTGTCCAGAAAGATCTGCCAAGACCATCAGAAGTAAATGAAACTATTCTAAGACCCTTAAAT GTAGAACCGCCTTTAACAGATTTACAGAAAAGTGAAGAACTAATCAAAAAAGAAATGATCACAATGCTTC ATTATGACCTTCTACATCACCCTTATGAACCATCTGGAAATAAAAAAGGCAAAACTGTAGGGTTTGGTAC CAATAATTCAGAGCACATTACCTATCTGGAACATAATCCTTATGAAAAGTTCTCCAAAGAAGAGCTGAAA AAGGCCCAGGATGTTTTGGTGCAGGAGATGGAAGTGGTTAAACAAGGAATGAGCCATGGAGAGCTCTCAA GTGAAGCTTATAACCAGGTGTGGGAAGAATGCTACAGTCAAGTTTTATATCTTCCTGGGCAGAGCCGCTA CACACGGGCCAATCTGGCTAGTAAAAAGGACAGAATTGAATCACTTGAAAAGAGGCTCGAGATAAACAGG GGTCACATGACGACAGAAGCCAAGAGGGCTGCAAAGATGGAAAAGAAGATGAAAATTTTGCTTGGGGGTT ACCAGTCTCGTGCTATGGGGCTCATGAAACAGTTGAATGACTTATGGGACCAAATTGAACAGGCTCACTT GGAGTTACGCACTTTTGAAGAACTCAAGAAACATGAAGATTCTGCTATTCCCCGGAGGCTAGAGTGTCTA AAAGAAGACGTTCAGCGACAACAAGAAAGAGAAAAGGAACTTCAACATAGATATGCTGATTTGCTGCTGG AGAAAGAGACTTTAAAGTCAAAATTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201702 protein sequence
Red=Cloning site Green=Tags(s) MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEK LLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDPRKLKPGEIDPNPETKPARPD PIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKRKRGVDY NAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAIL QTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVAL RTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGA EGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDF EIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLN VEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELK KAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINR GHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECL KEDVQRQQEREKELQHRYADLLLEKETLKSKF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001253 |
ORF Size | 2406 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001253.1, NM_001253.2, NM_001253.3, NP_001244.1 |
RefSeq Size | 6432 bp |
RefSeq ORF | 2409 bp |
Locus ID | 988 |
Cytogenetics | 6p21.1 |
Domains | myb_DNA-binding |
Protein Families | Transcription Factors |
Protein Pathways | Spliceosome |
MW | 92.3 kDa |
Gene Summary | 'The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119335 | CDC5L (untagged)-Human CDC5 cell division cycle 5-like (S. pombe) (CDC5L) |
USD 310.00 |
|
SC322498 | CDC5L (untagged)-Human CDC5 cell division cycle 5-like (S. pombe) (CDC5L) |
USD 310.00 |
|
RG201702 | CDC5L (GFP-tagged) - Human CDC5 cell division cycle 5-like (S. pombe) (CDC5L) |
USD 460.00 |
|
RC201702L1 | Lenti ORF clone of Human CDC5 cell division cycle 5-like (S. pombe) (CDC5L), Myc-DDK-tagged |
USD 768.00 |
|
RC201702L2 | Lenti ORF clone of Human CDC5 cell division cycle 5-like (S. pombe) (CDC5L), mGFP tagged |
USD 768.00 |
|
RC201702L3 | Lenti ORF clone of Human CDC5 cell division cycle 5-like (S. pombe) (CDC5L), Myc-DDK-tagged |
USD 620.00 |
|
RC201702L4 | Lenti ORF clone of Human CDC5 cell division cycle 5-like (S. pombe) (CDC5L), mGFP tagged |
USD 620.00 |
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